TY - JOUR
T1 - Nitrate reductase inactivating factor from rice cells in suspension culture
AU - Yamaya, Tomoyuki
AU - Ohira, Koji
PY - 1976/1/1
Y1 - 1976/1/1
N2 - With a crude extract of cultured rice cells, there was no direct relationship between the activity of NADH nitrate reductase measured and the amount of cell extract used, when the amount was large. It appeared that some factor in the cell extract inactivated nitrate reductase. The inactivating factor could be separated from nitrate reductase by (NH4)2SO4 precipitation. The factor seemed to be protein: 1) it was precipitable with (NH4)2SO4 heat labile, and pronase treatment caused loss of activity; 2) cycloheximide reduced the formation of the inactivating factor. The activity of this factor fluctuated during the growth period. The existence of this inactivating factor was further investigated in various other cultured cells.
AB - With a crude extract of cultured rice cells, there was no direct relationship between the activity of NADH nitrate reductase measured and the amount of cell extract used, when the amount was large. It appeared that some factor in the cell extract inactivated nitrate reductase. The inactivating factor could be separated from nitrate reductase by (NH4)2SO4 precipitation. The factor seemed to be protein: 1) it was precipitable with (NH4)2SO4 heat labile, and pronase treatment caused loss of activity; 2) cycloheximide reduced the formation of the inactivating factor. The activity of this factor fluctuated during the growth period. The existence of this inactivating factor was further investigated in various other cultured cells.
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U2 - 10.1093/oxfordjournals.pcp.a075319
DO - 10.1093/oxfordjournals.pcp.a075319
M3 - Article
AN - SCOPUS:15744367261
SN - 0032-0781
VL - 17
SP - 633
EP - 641
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 4
ER -