Nitric oxide inhibits the formation of advanced glycation end products

Koichi Asahi, Kohji Ichimori, Hiroe Nakazawa, Yuko Izuhara, Reiko Inagi, Tsuyoshi Watanabe, Toshio Miyata, Kiyoshi Kurokawa

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43 Citations (Scopus)


Background. Advanced glycation end products (AGEs) are elevated in renal failure and have been implicated in the pathogenesis of several uremic complications. Their formation is closely associated with oxidative stress. The recent observation that nitric oxide (NO) has an antioxidant effect led us to examine the possible role of NO in the generation of AGEs. Methods. We examined the effect of NO donors, 2,2'-(hydroxynitrosohydrazono)bis-ethanamine (NOC18) and S-nitroso-N-acetyl-DL-penicillamine (SNAP), on the in vitro formation of pentosidine, which was used as a surrogate marker for AGEs. Bovine serum albumin was incubated under air at 37°C in a medium containing either several AGE precursors or uremic plasma. To elucidate further the mechanism of the NO effect on AGE formation, we examined the generation of free radicals and carbonyls in pentose-driven pentosidine formation. Results. NO donors significantly inhibit the formation of pentosidine in a dose-dependent manner. The effect is abolished by the addition of a NO scavenging agent, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (carboxy-PTIO). The inhibitory effect results from NO but not from the NO donor molecule. It is best explained by the ability of NO to scavenge carbon-centered radicals, hydroxyl radical, and carbonyl compounds. Conclusions. NO inhibits pentosidine formation by scavenging free radicals and by inhibiting carbonyl compound formation. NO might be implicated in the atherogenic and inflammatory effects of AGEs: Reduced NO production and increased oxidative stress associated with atherosclerotic lesions may accelerate AGE formation and, thus, exacerbate endothelial dysfunction and accelerate the development of atherosclerosis in uremia.

Original languageEnglish
Pages (from-to)1780-1787
Number of pages8
JournalKidney International
Issue number4
Publication statusPublished - 2000


  • Anti-oxidant
  • Chronic renal failure
  • Glycoxidation
  • NOC18
  • Pentosidine
  • SNAP
  • Uremia


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