Non-invasive measurement of glucose uptake of skeletal muscle tissue models using a glucose nanobiosensor

Raquel Obregón, Samad Ahadian, Javier Ramón-Azcón, Luyang Chen, Takeshi Fujita, Hitoshi Shiku, Mingwei Chen, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Skeletal muscle tissues play a significant role to maintain the glucose level of whole body and any dysfunction of this tissue leads to the diabetes disease. A culture medium was created in which the muscle cells could survive for a long time and meanwhile it did not interfere with the glucose sensing. We fabricated a model of skeletal muscle tissues in vitro to monitor its glucose uptake. A nanoporous gold as a high sensitive nanobiosensor was then successfully developed and employed to detect the glucose uptake of the tissue models in this medium upon applying the electrical stimulation in a rapid, and non-invasive approach. The response of the glucose sensor was linear in a wide concentration range of 1-50. mM, with a detection limit of 3. μM at a signal-to-noise ratio of 3.0. The skeletal muscle tissue was electrically stimulated during 24. h and glucose uptake was monitored during this period. During the first 3. h of stimulation, electrically stimulated muscle tissue consumed almost twice the amount of glucose than counterpart non-stimulated sample. In total, the glucose consumption of muscle tissues was higher for the electrically stimulated tissues compared to those without applying the electrical field.

Original languageEnglish
Pages (from-to)194-201
Number of pages8
JournalBiosensors and Bioelectronics
Publication statusPublished - 2013 Dec 15


  • Diabetes mellitus
  • Electrical stimulation
  • Engineered skeletal muscle tissues
  • Glucose nanobiosensor
  • Glucose uptake


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