Novel Cross-domain Symbiosis between Candidatus Patescibacteria and Hydrogenotrophic Methanogenic Archaea Methanospirillum Discovered in a Methanogenic Ecosystem

Kyohei Kuroda, Kengo Kubota, Shuka Kagemasa, Ryosuke Nakai, Yuga Hirakata, Kyosuke Yamamoto, Masaru K. Nobu, Takashi Narihiro

Research output: Contribution to journalArticlepeer-review

Abstract

To identify novel cross-domain symbiosis between Candidatus Patescibacteria and Archaea, we performed fluorescence in situ hybridization (FISH) on enrichment cultures derived from methanogenic bioreactor sludge with the newly designed 32-520-1066 probe targeting the family-level uncultured clade 32-520/UBA5633 lineage in the class Ca. Paceibacteria. All FISH-detectable 32-520/UBA5633 cells were attached to Methanospirillum, indicating high host specificity. Transmission electron microscopy observations revealed 32-520/UBA5633-like cells that were specifically adherent to the plug structure of Methanospirillum-like rod-shaped cells. The metagenome-assembled genomes of 32-520/UBA5633 encoded unique gene clusters comprising pilin signal peptides and type IV pilins. These results provide novel insights into unseen symbiosis between Ca. Patescibacteria and Archaea.

Original languageEnglish
Article numberME22063
JournalMicrobes and environments
Volume37
Issue number4
DOIs
Publication statusPublished - 2022

Keywords

  • 32-520/UBA5633
  • Candidate Phyla Radiation (CPR) superphylum
  • Candidatus Patescibacteria
  • Methanospirillum
  • cross-domain symbiosis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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