TY - JOUR
T1 - Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica
AU - Mizutani, Tetsuya
AU - Sayama, Yusuke
AU - Nakanishi, Akira
AU - Ochiai, Hideharu
AU - Sakai, Kouji
AU - Wakabayashi, Kouji
AU - Tanaka, Nozomi
AU - Miura, Emi
AU - Oba, Mami
AU - Kurane, Ichiro
AU - Saijo, Masayuki
AU - Morikawa, Shigeru
AU - Ono, Shin ichi
N1 - Funding Information:
We thank Ms. Momoko Ogata of the National Institute of Infectious Diseases of Japan for her assistance. This study was supported in part by a grant from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (# 21580235 Scientific Research C:general). We are very grateful to the advice of Dr. Hiritaka Konuma, Tokai University, Drs. Hirokazu Kimura and Haruo Watanabe, National Institute of Infectious Diseases.
PY - 2011/3/30
Y1 - 2011/3/30
N2 - Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral genome sequences. In this study, viral DNA fragments were obtained using RDV, and approximately 15-kbp circular full genome sequences were determined using a next-generation sequencing system, overlapping PCR, and Southern blot analysis. One open reading frame (ORF) was homologous to the large T-antigen of polyomavirus; other ORFs have no homology with any nucleic or amino acid sequences of polyomavirus. Therefore, as this DNA virus might comprise a novel virus family, we provisionally named it Japanese eel endothelial cells-infecting virus (JEECV). JEECV was detected in both naturally and experimentally infected eels, suggesting that JEECV potentially causes VECNE.
AB - Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral genome sequences. In this study, viral DNA fragments were obtained using RDV, and approximately 15-kbp circular full genome sequences were determined using a next-generation sequencing system, overlapping PCR, and Southern blot analysis. One open reading frame (ORF) was homologous to the large T-antigen of polyomavirus; other ORFs have no homology with any nucleic or amino acid sequences of polyomavirus. Therefore, as this DNA virus might comprise a novel virus family, we provisionally named it Japanese eel endothelial cells-infecting virus (JEECV). JEECV was detected in both naturally and experimentally infected eels, suggesting that JEECV potentially causes VECNE.
KW - Anguilla japonica
KW - Japanese eel endothelial cells-infecting virus
KW - Novel DNA virus
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U2 - 10.1016/j.virol.2010.12.057
DO - 10.1016/j.virol.2010.12.057
M3 - Article
C2 - 21277610
AN - SCOPUS:79952535181
SN - 0042-6822
VL - 412
SP - 179
EP - 187
JO - Virology
JF - Virology
IS - 1
ER -