TY - JOUR
T1 - Novel monoclonal antibodies recognizing different subsets of lymphocytes from the common marmoset (Callithrix jacchus)
AU - Ito, Ryoji
AU - Maekawa, Shin ichiro
AU - Kawai, Kenji
AU - Suemizu, Hiroshi
AU - Suzuki, Shuzo
AU - Ishii, Hajime
AU - Tanioka, Yoshikuni
AU - Satake, Masanobu
AU - Yagita, Hideo
AU - Habu, Sonoko
AU - Ito, Mamoru
N1 - Funding Information:
We would like to thank Drs. Kenzaburo Tani and Ryo Kurita (Kyushu University, Japan), for the kind gift of marmoset CD45 cDNA, Dr. Erika Sasaki for her valuable advice, Natsuko Eguchi for maintenance of hybridomas, and Michiko Kamioka and Fumika Toyoda for breeding and maintenance of common marmosets. This work was partly supported by a Grant-in Aid from the Japanese Ministry of Education, Culture, Sports, Science and Technology and by the Japan Science and Technology Agency (JST).
PY - 2008/12/22
Y1 - 2008/12/22
N2 - Callithrix jacchus, the common marmoset, is a small new world primate that is considered effective as an experimental animal model for various human diseases. In this study, we generated monoclonal antibodies (mAbs) against common marmoset lymphocytes for immunological studies on the common marmoset. We established five hybridoma clones, 6C9, 10D7, 6F10, 7A4 and 5A1, producing anti-marmoset mAbs against cell surface antigens on marmoset T and/or B lymphocytes. We confirmed that 6C9 and 10D7 antibodies recognized CD45 antigen, and 6F10 antibody recognized CD8 antigen by flow cytometry using marmoset cDNA transfectants. We also tested them for application of immunoprecipitation, Western blot analysis and immunohistochemistry. We found that immunohistochemistry using marmoset spleen sections could be applied with all established mAbs but immunoprecipitation and the Western blot analysis could be applied with 6F10 and 10D7 antibodies but not with the other three mAbs. These results show that these monoclonal antibodies are useful for advancing immunological research on the common marmoset.
AB - Callithrix jacchus, the common marmoset, is a small new world primate that is considered effective as an experimental animal model for various human diseases. In this study, we generated monoclonal antibodies (mAbs) against common marmoset lymphocytes for immunological studies on the common marmoset. We established five hybridoma clones, 6C9, 10D7, 6F10, 7A4 and 5A1, producing anti-marmoset mAbs against cell surface antigens on marmoset T and/or B lymphocytes. We confirmed that 6C9 and 10D7 antibodies recognized CD45 antigen, and 6F10 antibody recognized CD8 antigen by flow cytometry using marmoset cDNA transfectants. We also tested them for application of immunoprecipitation, Western blot analysis and immunohistochemistry. We found that immunohistochemistry using marmoset spleen sections could be applied with all established mAbs but immunoprecipitation and the Western blot analysis could be applied with 6F10 and 10D7 antibodies but not with the other three mAbs. These results show that these monoclonal antibodies are useful for advancing immunological research on the common marmoset.
KW - Common marmoset
KW - Cross-reactivity
KW - Lymphocyte subset
KW - Monoclonal antibody
KW - New world primate
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U2 - 10.1016/j.imlet.2008.09.007
DO - 10.1016/j.imlet.2008.09.007
M3 - Article
C2 - 18977390
AN - SCOPUS:57449092504
SN - 0165-2478
VL - 121
SP - 116
EP - 122
JO - Immunology Letters
JF - Immunology Letters
IS - 2
ER -
