TY - JOUR
T1 - Novel PCR-mediated mutagenesis employing DNA containing a natural abasic site as a template and translesional Taq DNA polymerase
AU - Kobayashi, Atsushi
AU - Kitaoka, Motomitsu
AU - Hayashi, Kiyoshi
N1 - Funding Information:
This work was supported by a grant from the Program for the Promotion of Basic Research Activities for Innovative Biosciences.
PY - 2005/3/30
Y1 - 2005/3/30
N2 - We describe a novel method of PCR-mediated mutagenesis employing DNA containing a natural abasic site and translesional Taq DNA polymerase. This method incorporated an adenine (80.8%) or guanine (7.7%) residue or led to a base deletion mutation (11.2%) opposite the abasic site. We conclude that the combination of DNA containing an abasic site and translesional Taq DNA polymerase is an easy and useful technique for PCR-mediated mutagenesis, having advantages different from those of conventional error-prone PCR.
AB - We describe a novel method of PCR-mediated mutagenesis employing DNA containing a natural abasic site and translesional Taq DNA polymerase. This method incorporated an adenine (80.8%) or guanine (7.7%) residue or led to a base deletion mutation (11.2%) opposite the abasic site. We conclude that the combination of DNA containing an abasic site and translesional Taq DNA polymerase is an easy and useful technique for PCR-mediated mutagenesis, having advantages different from those of conventional error-prone PCR.
KW - Abasic site
KW - Base-selective substitution mutagenesis
KW - PCR-mediated mutagenesis
KW - Translesional DNA polymerase
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U2 - 10.1016/j.jbiotec.2004.10.016
DO - 10.1016/j.jbiotec.2004.10.016
M3 - Article
C2 - 15707683
AN - SCOPUS:13544262556
SN - 0168-1656
VL - 116
SP - 227
EP - 232
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 3
ER -