Nuclear Lamin is required for Winged Eye-mediated transdetermination of Drosophila imaginal disc

Keita Masuko, Hirofumi Furuhashi, Kanae Komaba, Eriko Numao, Rumi Nakajima, Naoyuki Fuse, Shoichiro Kurata

Research output: Contribution to journalArticlepeer-review


Drosophila imaginal discs often change their cell fate under stress conditions, and this phenomenon, called transdetermination (TD), has long been a useful model for studying cell fate plasticity during regeneration. We previously identified a chromatin-associated protein, Winged Eye (Wge), which induces eye-to-wing TD upon its over-expression in eye imaginal discs. However, the molecular mechanism of Wge-mediated TD remains obscure. Here, we analyzed Wge-interacting proteins and found that several heterochromatin-related proteins, including a nuclear lamina protein, Lamin (Lam), were associated with Wge protein in cultured cells. Knockdown experiments revealed that Lam is indeed required for Wge-mediated eye-to-wing TD. Moreover, Wge over-expression altered the spatial organization of genomic DNA inside the cell nuclei. Accordingly, we suggest that Wge interacts with Lam to link some genomic regions with the nuclear periphery and regulates chromatin dynamics in imaginal disc TD.

Original languageEnglish
Pages (from-to)724-731
Number of pages8
JournalGenes to Cells
Issue number8
Publication statusPublished - 2018 Aug


  • Drosophila
  • Lamin
  • Winged Eye
  • cell fate
  • imaginal disc
  • reprogramming
  • transdetermination

ASJC Scopus subject areas

  • Genetics
  • Cell Biology


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