TY - JOUR
T1 - Nuclear localization and involvement in DNA synthesis of Sarcophaga prolyl endopeptidase
AU - Ohtsuki, Sumio
AU - Homma, Ko Ichi
AU - Kurata, Shoichiro
AU - Natori, Shunji
PY - 1997/6
Y1 - 1997/6
N2 - A specific prolyl endopeptidase (PEP) inhibitor, ZTTA, selectively inhibited DNA synthesis by imaginal discs and cultured embryonic cells of Sarcophaga peregrina (flesh fly). PEP was found to be localized in restricted nuclear regions. Unfertilized eggs were shown to contain a maternal message for PEP and analysis of Sarcophaga embryos at blastdermal stage revealed PEP was localized exclusively in the nuclei. These results suggest that PEP participates in DNA synthesis by, and therefore cell proliferation, of insect cells. This is the first demonstration of a biological function of PEP.
AB - A specific prolyl endopeptidase (PEP) inhibitor, ZTTA, selectively inhibited DNA synthesis by imaginal discs and cultured embryonic cells of Sarcophaga peregrina (flesh fly). PEP was found to be localized in restricted nuclear regions. Unfertilized eggs were shown to contain a maternal message for PEP and analysis of Sarcophaga embryos at blastdermal stage revealed PEP was localized exclusively in the nuclei. These results suggest that PEP participates in DNA synthesis by, and therefore cell proliferation, of insect cells. This is the first demonstration of a biological function of PEP.
KW - DNA synthesis
KW - Imaginal disc
KW - Nuclear localization
KW - Prolyl endopeptidase
KW - Sarcophaga peregrina
UR - http://www.scopus.com/inward/record.url?scp=0030759451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030759451&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.jbchem.a021712
DO - 10.1093/oxfordjournals.jbchem.a021712
M3 - Article
C2 - 9354394
AN - SCOPUS:0030759451
SN - 0021-924X
VL - 121
SP - 1176
EP - 1181
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 6
ER -