Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

Hiroyasu Kitashiba; Yusuke Ban; Chikako Honda; Takaya Moriguchi

doi:10.5511/plantbiotechnology.23.515

Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

Hiroyasu Kitashiba, Yusuke Ban, Chikako Honda, Takaya Moriguchi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively.

Original language	English
Pages (from-to)	515-518
Number of pages	4
Journal	Plant Biotechnology
Volume	23
Issue number	5
DOIs	https://doi.org/10.5511/plantbiotechnology.23.515
Publication status	Published - 2006

Keywords

DREB/CBF
Green fluorescence protein
Nuclear localization signal
Sweet cherry

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Plant Science

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title = "Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter",

abstract = "The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively.",

keywords = "DREB/CBF, Green fluorescence protein, Nuclear localization signal, Sweet cherry",

author = "Hiroyasu Kitashiba and Yusuke Ban and Chikako Honda and Takaya Moriguchi",

year = "2006",

doi = "10.5511/plantbiotechnology.23.515",

language = "English",

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pages = "515--518",

journal = "Plant Biotechnology",

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T1 - Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

AU - Kitashiba, Hiroyasu

AU - Ban, Yusuke

AU - Honda, Chikako

AU - Moriguchi, Takaya

PY - 2006

Y1 - 2006

N2 - The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively.

AB - The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively.

KW - DREB/CBF

KW - Green fluorescence protein

KW - Nuclear localization signal

KW - Sweet cherry

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M3 - Article

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JO - Plant Biotechnology

JF - Plant Biotechnology

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Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

Abstract

Keywords

ASJC Scopus subject areas

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