Nutrient concentration-sensitive microorganism-based biosensor

Carl Frederik Werner, Simone Groebel, Christoph Krumbe, Torsten Wagner, Thorsten Selmer, Tatsuo Yoshinobu, Marcus E.M. Baumann, Michael Keusgen, Michael J. Schöning

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


This work presents a biosensor based on living cells immobilised on a light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as nutrient and Escherichia coli as microorganism. The function of this biosensor is comparable to an enzyme-based field-effect sensor when using diffusion limitation. With the help of addressability of the LAPS an on-chip differential set-up was developed. Thus, external influences such as sensor drift, temperature and external pH changes can be minimised.

Original languageEnglish
Pages (from-to)900-904
Number of pages5
JournalPhysica Status Solidi (A) Applications and Materials Science
Issue number5
Publication statusPublished - 2012 May


  • biosensor
  • extracellular acidification
  • glucose
  • light-addressable potentiometric sensor


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