Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope

Motoi Nakao; Shinji Inoue; Ryu Oishi; Tatsuo Yoshinobu; Hiroshi Iwasaki

doi:10.1016/0922-338X(95)94085-6

Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope

Motoi Nakao, Shinji Inoue, Ryu Oishi, Tatsuo Yoshinobu, Hiroshi Iwasaki

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results.

Original language	English
Pages (from-to)	163-166
Number of pages	4
Journal	Journal of Fermentation and Bioengineering
Volume	79
Issue number	2
DOIs	https://doi.org/10.1016/0922-338X(95)94085-6
Publication status	Published - 1995
Externally published	Yes

Keywords

colony-counting
metabolism
microorganism
pH sensor
pH-imaging sensor

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/0922-338X(95)94085-6

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title = "Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope",

abstract = "The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results.",

keywords = "colony-counting, metabolism, microorganism, pH sensor, pH-imaging sensor",

author = "Motoi Nakao and Shinji Inoue and Ryu Oishi and Tatsuo Yoshinobu and Hiroshi Iwasaki",

note = "Funding Information: This work was supported by The Asahi Glass Foundation, and also by a Grant-in-Aid for Developmental Scientific Research (contract No. 05558113) from the Japanese Ministry of Education, Science, and Culture. The authors are grateful to Mr. Y. Hirofuji at Matsushita Electric Industrial Co. Ltd. for preparing of the sensor wafers and Mr. T. Hirose of Nomura Mi&Scie&e Co. Ltd. for supplying P. diminuta (ATCCl9146).",

year = "1995",

doi = "10.1016/0922-338X(95)94085-6",

language = "English",

volume = "79",

pages = "163--166",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "Elsevier",

number = "2",

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TY - JOUR

T1 - Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope

AU - Nakao, Motoi

AU - Inoue, Shinji

AU - Oishi, Ryu

AU - Yoshinobu, Tatsuo

AU - Iwasaki, Hiroshi

N1 - Funding Information: This work was supported by The Asahi Glass Foundation, and also by a Grant-in-Aid for Developmental Scientific Research (contract No. 05558113) from the Japanese Ministry of Education, Science, and Culture. The authors are grateful to Mr. Y. Hirofuji at Matsushita Electric Industrial Co. Ltd. for preparing of the sensor wafers and Mr. T. Hirose of Nomura Mi&Scie&e Co. Ltd. for supplying P. diminuta (ATCCl9146).

PY - 1995

Y1 - 1995

N2 - The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results.

AB - The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results.

KW - colony-counting

KW - metabolism

KW - microorganism

KW - pH sensor

KW - pH-imaging sensor

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U2 - 10.1016/0922-338X(95)94085-6

DO - 10.1016/0922-338X(95)94085-6

M3 - Article

AN - SCOPUS:0028902606

SN - 1389-1723

VL - 79

SP - 163

EP - 166

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 2

ER -

Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope

Abstract

Keywords

ASJC Scopus subject areas

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