Observation of the three-dimensional structure of actin bundles formed with polycations

Kazuhiro Shikinaka; Hyuckjoon Kwon; Akira Kakugo; Hidemitsu Furukawa; Yoshihito Osada; Jian Ping Gong; Yoshitaka Aoyama; Hideo Nishioka; Hiroshi Jinnai; Takaharu Okajima

doi:10.1021/bm701068n

Observation of the three-dimensional structure of actin bundles formed with polycations

Kazuhiro Shikinaka, Hyuckjoon Kwon, Akira Kakugo, Hidemitsu Furukawa, Yoshihito Osada, Jian Ping Gong, Yoshitaka Aoyama, Hideo Nishioka, Hiroshi Jinnai, Takaharu Okajima

IMRAM - Division of Measurements

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18 Citations (Scopus)

Abstract

Three-dimensional structures of actin bundles formed with polycations were observed by using transmission electron microtomography and atomic force microscopy. We found, for the first time, that the cross-sectional morphology of actin bundles depends on the polycation species and ionic strength, while it is insensitive to the degree of polymerization and concentration of polycation. Actin bundles formed with poly-N-[3-(dimethylamino)propyl] acrylamide methyl chloride quaternary show a ribbon-like cross-sectional morphology in low salt concentrations that changes to cylindrical cross-sectional morphology with hexagonal packing of the actin filaments in high salt concentrations. Contrastingly, actin bundles formed with POly-L-lysine show triangular cross-sectional morphology with hexagonal packing of the actin filaments. These variations in cross-sectional morphology are discussed in terms of anisotropy in the electrostatic energy barrier.

Original language	English
Pages (from-to)	537-542
Number of pages	6
Journal	Biomacromolecules
Volume	9
Issue number	2
DOIs	https://doi.org/10.1021/bm701068n
Publication status	Published - 2008 Feb

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title = "Observation of the three-dimensional structure of actin bundles formed with polycations",

abstract = "Three-dimensional structures of actin bundles formed with polycations were observed by using transmission electron microtomography and atomic force microscopy. We found, for the first time, that the cross-sectional morphology of actin bundles depends on the polycation species and ionic strength, while it is insensitive to the degree of polymerization and concentration of polycation. Actin bundles formed with poly-N-[3-(dimethylamino)propyl] acrylamide methyl chloride quaternary show a ribbon-like cross-sectional morphology in low salt concentrations that changes to cylindrical cross-sectional morphology with hexagonal packing of the actin filaments in high salt concentrations. Contrastingly, actin bundles formed with POly-L-lysine show triangular cross-sectional morphology with hexagonal packing of the actin filaments. These variations in cross-sectional morphology are discussed in terms of anisotropy in the electrostatic energy barrier.",

author = "Kazuhiro Shikinaka and Hyuckjoon Kwon and Akira Kakugo and Hidemitsu Furukawa and Yoshihito Osada and Gong, {Jian Ping} and Yoshitaka Aoyama and Hideo Nishioka and Hiroshi Jinnai and Takaharu Okajima",

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T1 - Observation of the three-dimensional structure of actin bundles formed with polycations

AU - Shikinaka, Kazuhiro

AU - Kwon, Hyuckjoon

AU - Kakugo, Akira

AU - Furukawa, Hidemitsu

AU - Osada, Yoshihito

AU - Gong, Jian Ping

AU - Aoyama, Yoshitaka

AU - Nishioka, Hideo

AU - Jinnai, Hiroshi

AU - Okajima, Takaharu

PY - 2008/2

Y1 - 2008/2

N2 - Three-dimensional structures of actin bundles formed with polycations were observed by using transmission electron microtomography and atomic force microscopy. We found, for the first time, that the cross-sectional morphology of actin bundles depends on the polycation species and ionic strength, while it is insensitive to the degree of polymerization and concentration of polycation. Actin bundles formed with poly-N-[3-(dimethylamino)propyl] acrylamide methyl chloride quaternary show a ribbon-like cross-sectional morphology in low salt concentrations that changes to cylindrical cross-sectional morphology with hexagonal packing of the actin filaments in high salt concentrations. Contrastingly, actin bundles formed with POly-L-lysine show triangular cross-sectional morphology with hexagonal packing of the actin filaments. These variations in cross-sectional morphology are discussed in terms of anisotropy in the electrostatic energy barrier.

AB - Three-dimensional structures of actin bundles formed with polycations were observed by using transmission electron microtomography and atomic force microscopy. We found, for the first time, that the cross-sectional morphology of actin bundles depends on the polycation species and ionic strength, while it is insensitive to the degree of polymerization and concentration of polycation. Actin bundles formed with poly-N-[3-(dimethylamino)propyl] acrylamide methyl chloride quaternary show a ribbon-like cross-sectional morphology in low salt concentrations that changes to cylindrical cross-sectional morphology with hexagonal packing of the actin filaments in high salt concentrations. Contrastingly, actin bundles formed with POly-L-lysine show triangular cross-sectional morphology with hexagonal packing of the actin filaments. These variations in cross-sectional morphology are discussed in terms of anisotropy in the electrostatic energy barrier.

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Observation of the three-dimensional structure of actin bundles formed with polycations

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