TY - JOUR
T1 - Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells
AU - Shibuya, Isao
AU - Yoshimura, Kentaro
AU - Miyamoto, Yoichi
AU - Yamada, Atsushi
AU - Takami, Masamichi
AU - Suzawa, Tetsuo
AU - Suzuki, Dai
AU - Ikumi, Noriharu
AU - Hiura, Fumitaka
AU - Anada, Takahisa
AU - Suzuki, Osamu
AU - Kamijo, Ryutaro
N1 - Funding Information:
This study was supported in part by Grants-in-Aid for Scientific Research from The Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) (23106010) and the Japan Society for the Promotion of Sciences (21592372, 22890181, 23390450, 23592748, 23659909) and by grants from the Project to Establish Strategic Research Center for Innovative Dentistry by MEXT (S0801016, S1001010).
PY - 2013/5
Y1 - 2013/5
N2 - Implantation of octacalcium phosphate (OCP), a hydroxyapatite precursor, has been reported to induce chondrogenesis in vivo. In this study, we examined the effects of OCP on the chondrogenic differentiation of mouse chondroblastic ATDC5 cells in vitro. Contrary to our expectation, chondrogenic differentiation of ATDC5 cells evaluated by the mRNA expression of Col2a1, Acan and Col10a1 was suppressed by OCP. Among Sox9, Sox5 and Sox6, essential transcription factors for chondrogenesis, the expression of Sox6 mRNA was markedly lowered by OCP. Whereas ATDC5 cells dissolved OCP to liberate calcium and inorganic phosphorus, increased calcium or phosphate in the medium had little effect on the differentiation of these cells. Direct contact of ATDC5 cells with OCP was required to suppress the expression of Col2a1 and Sox6 mRNAs, whereas the introduction of Sox6 short interfering RNA lowered the expression of Col2a1 mRNA. On the other hand, the forced expression of Sox6 protein partially but significantly, restored the expression of Col2a1 mRNA suppressed by OCP. These results indicate that OCP suppresses the chondrogenic differentiation of ATDC5 cells, at least in part, at the Sox6 transcription level.
AB - Implantation of octacalcium phosphate (OCP), a hydroxyapatite precursor, has been reported to induce chondrogenesis in vivo. In this study, we examined the effects of OCP on the chondrogenic differentiation of mouse chondroblastic ATDC5 cells in vitro. Contrary to our expectation, chondrogenic differentiation of ATDC5 cells evaluated by the mRNA expression of Col2a1, Acan and Col10a1 was suppressed by OCP. Among Sox9, Sox5 and Sox6, essential transcription factors for chondrogenesis, the expression of Sox6 mRNA was markedly lowered by OCP. Whereas ATDC5 cells dissolved OCP to liberate calcium and inorganic phosphorus, increased calcium or phosphate in the medium had little effect on the differentiation of these cells. Direct contact of ATDC5 cells with OCP was required to suppress the expression of Col2a1 and Sox6 mRNAs, whereas the introduction of Sox6 short interfering RNA lowered the expression of Col2a1 mRNA. On the other hand, the forced expression of Sox6 protein partially but significantly, restored the expression of Col2a1 mRNA suppressed by OCP. These results indicate that OCP suppresses the chondrogenic differentiation of ATDC5 cells, at least in part, at the Sox6 transcription level.
KW - Chondrocyte
KW - Differentiation
KW - Octacalcium phosphate
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U2 - 10.1007/s00441-012-1548-8
DO - 10.1007/s00441-012-1548-8
M3 - Article
C2 - 23274552
AN - SCOPUS:84880570878
SN - 0302-766X
VL - 352
SP - 401
EP - 412
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 2
ER -