Ocular and systemic factors affecting laser speckle flowgraphy measurements in the optic nerve head

Ayako Anraku, Nobuko Enomoto, Goji Tomita, Aiko Iwase, Takashi Sato, Nobuyuki Shoji, Tomoaki Shiba, Toru Nakazawa, Kazuhisa Sugiyama, Koji Nitta, Makoto Araie

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Purpose: To investigate the ocular and systemic factors related to glaucoma and to be adjusted for interindividual comparison of ocular blood flow measurement results by laser speckle flowgraphy (LSFG) obtained from the optic nerve head (ONH) in normal Japanese individuals. Methods: A multicenter, prospective cross-sectional study was conducted. The ONH tissue-area and vessel-area mean blur rate (MT and MV) were evaluated using LSFG and ONH structural parameters using planimetric methods. Multivariate linear mixed-effects modeled regression analysis was used to identify the contributing factors to the MT and MV. The explanatory variables were age; gender; smoking history; body mass index; mean arterial pressure (MAP); heart rate; intraocular pressure; axial length (AL); disc, rim, cup, and β-peripapillary atrophy (β-PPA) areas; and central retinal artery and vein equivalents. Results: In total, 195 eyes of 126 healthy individuals with an average age of 48.1 years were included. Multivariate analysis showed that MAP and disc area had a negative (P < 0.001) correlation, whereas β-PPA area had a positive correlation with MT (P = 0.010). Age and AL had a negative correlation (P = 0.001 and P = 0.011, respectively), whereas cup area had a positive correlation (P = 0.012) with MV. Conclusions: Interindividual comparison of MT or MV must be adjusted for both systemic factors (blood pressure or age) and local ocular factors (AL and disc, cup, or β-PPA area). Translational Relevance: Our results provided reference data on the LSFG measurement and are important in comparing ocular blood flow between individuals using LSFG.

Original languageEnglish
Article number13
Pages (from-to)1-12
Number of pages12
JournalTranslational Vision Science and Technology
Issue number1
Publication statusPublished - 2021


  • Blood flow
  • Disc parameter
  • Laser speckle flowgraphy
  • Normal subjects
  • Optic nerve head

ASJC Scopus subject areas

  • Biomedical Engineering
  • Ophthalmology


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