Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Michihiro Sugahara; Changyong Song; Mamoru Suzuki; Tetsuya Masuda; Shigeyuki Inoue; Takanori Nakane; Fumiaki Yumoto; Eriko Nango; Rie Tanaka; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Takaki Hatsui; Makina Yabashi; Osamu Nureki; Keiji Numata; So Iwata

doi:10.1038/srep24484

Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Michihiro Sugahara, Changyong Song, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Takanori Nakane, Fumiaki Yumoto, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, So Iwata

IMRAM - Division of Organic- and Biomaterials Research

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.

Original language	English
Article number	24484
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep24484
Publication status	Published - 2016 Apr 18

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abstract = "The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-{\AA} resolution.",

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AU - Sugahara, Michihiro

AU - Song, Changyong

AU - Suzuki, Mamoru

AU - Masuda, Tetsuya

AU - Inoue, Shigeyuki

AU - Nakane, Takanori

AU - Yumoto, Fumiaki

AU - Nango, Eriko

AU - Tanaka, Rie

AU - Tono, Kensuke

AU - Joti, Yasumasa

AU - Kameshima, Takashi

AU - Hatsui, Takaki

AU - Yabashi, Makina

AU - Nureki, Osamu

AU - Numata, Keiji

AU - Iwata, So

PY - 2016/4/18

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Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Abstract

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