On-chip direct freezing and thawing of mammalian cells

Lei Li, Xiaoqing Lv, Hua Guo, Xuetao Shi, Jing Liu

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)


    Here we present a simple protocol for directly freezing and thawing mammalian cells on a PDMS-glass chip, which enables cell storage at -80 °C on a chip for several days to several months. Two different sizes of straight channels were used (channel I: 4 cm length, 2 mm width, 100 μm height; and channel II: 1 cm length, 900 μm width, 60 μm height). The cell suspension (cell pellet resuspended in freezing medium, which was composed of dimethylsulfoxide (DMSO), fetal bovine serum (FBS) and culture medium) was injected into the microchannel, and the chip was packed and put into the centrifuge tube filled with isopropyl alcohol. The centrifuge tubes were maintained at -80 °C in a cryogenic refrigerator until further use. We demonstrated that HUVECs, 3T3, SKBR3, and HepG2 cells were successfully thawed and grew in the microchannel after 10-days to 1-year cryopreservation by using this protocol. This journal is

    Original languageEnglish
    Pages (from-to)34443-34447
    Number of pages5
    JournalRSC Advances
    Issue number65
    Publication statusPublished - 2014

    ASJC Scopus subject areas

    • Chemistry(all)
    • Chemical Engineering(all)


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