TY - JOUR
T1 - On the cDNA's for two types of rat pancreatic secretory trypsin inhibitor
AU - Horii, Akira
AU - Tomita, Naohiro
AU - Yokouchi, Hideoki
AU - Doi, Sadayuki
AU - Uda, Kenichi
AU - Ogawa, Michio
AU - Mori, Takesada
AU - Matsubara, Kenichi
N1 - Funding Information:
We thank Dr. Y. Takahashi for his valuable advice and discussions and Miss K. Mimura for her secretarial assistance in preparing the manuscript. This work was supported by a Scientific Research Grant to K. M. from the Ministry of Education, Science and Culture, Japan.
PY - 1989/7/14
Y1 - 1989/7/14
N2 - Two types of cDNA, which code for the two types of rat pancreatic secretory trypsin inhibitors (PSTIs), were cloned and sequenced. Both predicted amino acid sequences consisting of 79 amino acids, with the secretion signal peptide consisting of 18 and 23 amino acids for PSTI-I and PSTI-II, respectively. The nucleotide sequences were 91% homologous between the two cDNAs, but 68% and 65% homologous, respectively, when compared with human PSTI cDNA. Northern blot analyses showed that PSTI-I is expressed in the pancreas, whereas PSTI-II is expressed in the pancreas and the liver using the same promotor. Southern blot analyses suggested that both PSTI-I and PSTI-II genes are single copy genes per haploid genome. Duplication of rat PSTI gene seems to have occurred recently, after the divergene of humans and rats.
AB - Two types of cDNA, which code for the two types of rat pancreatic secretory trypsin inhibitors (PSTIs), were cloned and sequenced. Both predicted amino acid sequences consisting of 79 amino acids, with the secretion signal peptide consisting of 18 and 23 amino acids for PSTI-I and PSTI-II, respectively. The nucleotide sequences were 91% homologous between the two cDNAs, but 68% and 65% homologous, respectively, when compared with human PSTI cDNA. Northern blot analyses showed that PSTI-I is expressed in the pancreas, whereas PSTI-II is expressed in the pancreas and the liver using the same promotor. Southern blot analyses suggested that both PSTI-I and PSTI-II genes are single copy genes per haploid genome. Duplication of rat PSTI gene seems to have occurred recently, after the divergene of humans and rats.
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U2 - 10.1016/0006-291X(89)91975-X
DO - 10.1016/0006-291X(89)91975-X
M3 - Article
C2 - 2751646
AN - SCOPUS:0024405314
SN - 0006-291X
VL - 162
SP - 151
EP - 159
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -