Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer

Suguru Fujisawa; Hiroshige Arakawa; Ryo Suzuki; Kazuo Maruyama; Tetsuya Kodama; Masahiro Yasunaga; Yoshikatsu Koga; Yasuhiro Matsumura

doi:10.4155/tde.10.23

Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer

Suguru Fujisawa, Hiroshige Arakawa, Ryo Suzuki, Kazuo Maruyama, Tetsuya Kodama, Masahiro Yasunaga, Yoshikatsu Koga, Yasuhiro Matsumura

MEN - Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Background: We investigated the effectiveness of ultrasound-mediated destruction of bubble liposome (UBL) for siRNA transfer by observing reduction in the luciferase activity of human bladder tumor RT-112 cells transfected with the luciferase gene (RT-112Luc) following luciferase siRNA transfer into the cells. Results: siRNA was transferred to 26% of RT-112Luc cells by UBL and the luciferase activity of RT-112Luc cells was significantly suppressed by UBL using the luciferase siRNA, compared with that using nonspecific siRNA in vitro (p = 0.036). The luciferase activity of RT-112Luc tumor was suppressed by UBL using luciferase siRNA compared with that using nonspecific siRNA 2 days after the in vivo treatment. Conclusion: This study showed that UBL is suitable for siRNA transfer to mammalian cells.

Original language	English
Pages (from-to)	247-255
Number of pages	9
Journal	Therapeutic Delivery
Volume	1
Issue number	2
DOIs	https://doi.org/10.4155/tde.10.23
Publication status	Published - 2010 Aug

ASJC Scopus subject areas

Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4155/tde.10.23

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title = "Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer",

abstract = "Background: We investigated the effectiveness of ultrasound-mediated destruction of bubble liposome (UBL) for siRNA transfer by observing reduction in the luciferase activity of human bladder tumor RT-112 cells transfected with the luciferase gene (RT-112Luc) following luciferase siRNA transfer into the cells. Results: siRNA was transferred to 26% of RT-112Luc cells by UBL and the luciferase activity of RT-112Luc cells was significantly suppressed by UBL using the luciferase siRNA, compared with that using nonspecific siRNA in vitro (p = 0.036). The luciferase activity of RT-112Luc tumor was suppressed by UBL using luciferase siRNA compared with that using nonspecific siRNA 2 days after the in vivo treatment. Conclusion: This study showed that UBL is suitable for siRNA transfer to mammalian cells.",

author = "Suguru Fujisawa and Hiroshige Arakawa and Ryo Suzuki and Kazuo Maruyama and Tetsuya Kodama and Masahiro Yasunaga and Yoshikatsu Koga and Yasuhiro Matsumura",

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pages = "247--255",

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T1 - Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer

AU - Fujisawa, Suguru

AU - Arakawa, Hiroshige

AU - Suzuki, Ryo

AU - Maruyama, Kazuo

AU - Kodama, Tetsuya

AU - Yasunaga, Masahiro

AU - Koga, Yoshikatsu

AU - Matsumura, Yasuhiro

PY - 2010/8

Y1 - 2010/8

N2 - Background: We investigated the effectiveness of ultrasound-mediated destruction of bubble liposome (UBL) for siRNA transfer by observing reduction in the luciferase activity of human bladder tumor RT-112 cells transfected with the luciferase gene (RT-112Luc) following luciferase siRNA transfer into the cells. Results: siRNA was transferred to 26% of RT-112Luc cells by UBL and the luciferase activity of RT-112Luc cells was significantly suppressed by UBL using the luciferase siRNA, compared with that using nonspecific siRNA in vitro (p = 0.036). The luciferase activity of RT-112Luc tumor was suppressed by UBL using luciferase siRNA compared with that using nonspecific siRNA 2 days after the in vivo treatment. Conclusion: This study showed that UBL is suitable for siRNA transfer to mammalian cells.

AB - Background: We investigated the effectiveness of ultrasound-mediated destruction of bubble liposome (UBL) for siRNA transfer by observing reduction in the luciferase activity of human bladder tumor RT-112 cells transfected with the luciferase gene (RT-112Luc) following luciferase siRNA transfer into the cells. Results: siRNA was transferred to 26% of RT-112Luc cells by UBL and the luciferase activity of RT-112Luc cells was significantly suppressed by UBL using the luciferase siRNA, compared with that using nonspecific siRNA in vitro (p = 0.036). The luciferase activity of RT-112Luc tumor was suppressed by UBL using luciferase siRNA compared with that using nonspecific siRNA 2 days after the in vivo treatment. Conclusion: This study showed that UBL is suitable for siRNA transfer to mammalian cells.

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Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer

Abstract

ASJC Scopus subject areas

UN SDGs

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