TY - JOUR
T1 - Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation
AU - Kawana, Yohei
AU - Imai, Junta
AU - Morizawa, Yosuke M.
AU - Ikoma, Yoko
AU - Kohata, Masato
AU - Komamura, Hiroshi
AU - Sato, Toshihiro
AU - Izumi, Tomohito
AU - Yamamoto, Junpei
AU - Endo, Akira
AU - Sugawara, Hiroto
AU - Kubo, Haremaru
AU - Hosaka, Shinichiro
AU - Munakata, Yuichiro
AU - Asai, Yoichiro
AU - Kodama, Shinjiro
AU - Takahashi, Kei
AU - Kaneko, Keizo
AU - Sawada, Shojiro
AU - Yamada, Tetsuya
AU - Ito, Akira
AU - Niizuma, Kuniyasu
AU - Tominaga, Teiji
AU - Yamanaka, Akihiro
AU - Matsui, Ko
AU - Katagiri, Hideki
N1 - Publisher Copyright:
© The Author(s) 2023. corrected publication 2024.
PY - 2024/7
Y1 - 2024/7
N2 - The enhancement of insulin secretion and of the proliferation of pancreatic β cells are promising therapeutic options for diabetes. Signals from the vagal nerve regulate both processes, yet the effectiveness of stimulating the nerve is unclear, owing to a lack of techniques for doing it so selectively and prolongedly. Here we report two optogenetic methods for vagal-nerve stimulation that led to enhanced glucose-stimulated insulin secretion and to β cell proliferation in mice expressing choline acetyltransferase-channelrhodopsin 2. One method involves subdiaphragmatic implantation of an optical fibre for the photostimulation of cholinergic neurons expressing a blue-light-sensitive opsin. The other method, which suppressed streptozotocin-induced hyperglycaemia in the mice, involves the selective activation of vagal fibres by placing blue-light-emitting lanthanide microparticles in the pancreatic ducts of opsin-expressing mice, followed by near-infrared illumination. The two methods show that signals from the vagal nerve, especially from nerve fibres innervating the pancreas, are sufficient to regulate insulin secretion and β cell proliferation.
AB - The enhancement of insulin secretion and of the proliferation of pancreatic β cells are promising therapeutic options for diabetes. Signals from the vagal nerve regulate both processes, yet the effectiveness of stimulating the nerve is unclear, owing to a lack of techniques for doing it so selectively and prolongedly. Here we report two optogenetic methods for vagal-nerve stimulation that led to enhanced glucose-stimulated insulin secretion and to β cell proliferation in mice expressing choline acetyltransferase-channelrhodopsin 2. One method involves subdiaphragmatic implantation of an optical fibre for the photostimulation of cholinergic neurons expressing a blue-light-sensitive opsin. The other method, which suppressed streptozotocin-induced hyperglycaemia in the mice, involves the selective activation of vagal fibres by placing blue-light-emitting lanthanide microparticles in the pancreatic ducts of opsin-expressing mice, followed by near-infrared illumination. The two methods show that signals from the vagal nerve, especially from nerve fibres innervating the pancreas, are sufficient to regulate insulin secretion and β cell proliferation.
UR - http://www.scopus.com/inward/record.url?scp=85179318335&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85179318335&partnerID=8YFLogxK
U2 - 10.1038/s41551-023-01113-2
DO - 10.1038/s41551-023-01113-2
M3 - Article
C2 - 37945752
AN - SCOPUS:85179318335
SN - 2157-846X
VL - 8
SP - 808
EP - 822
JO - Nature Biomedical Engineering
JF - Nature Biomedical Engineering
IS - 7
ER -