TY - JOUR
T1 - Oral Microbiome Analysis in Prospective Genome Cohort Studies of the Tohoku Medical Megabank Project
AU - Saito, Sakae
AU - Aoki, Yuichi
AU - Tamahara, Toru
AU - Goto, Maki
AU - Matsui, Hiroyuki
AU - Kawashima, Junko
AU - Danjoh, Inaho
AU - Hozawa, Atsushi
AU - Kuriyama, Shinichi
AU - Suzuki, Yoichi
AU - Fuse, Nobuo
AU - Kure, Shigeo
AU - Yamashita, Riu
AU - Tanabe, Osamu
AU - Minegishi, Naoko
AU - Kinoshita, Kengo
AU - Tsuboi, Akito
AU - Shimizu, Ritsuko
AU - Yamamoto, Masayuki
N1 - Funding Information:
This work was supported by Tohoku Medical Megabank Project from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and the Japan Agency for Medical Research and Development (AMED) under the grant numbers JP20km0105001 and JP20km0105002. It was also supported by the Advanced Genome Research and Bioinformatics Study to Facilitate Medical Innovation (GRIFIN) grant number JP20km0405203 and Facilitation of R&D Platform for AMED Genome Medicine Support conducted by AMED grant number JP20km0405001.
Publisher Copyright:
© Copyright © 2021 Saito, Aoki, Tamahara, Goto, Matsui, Kawashima, Danjoh, Hozawa, Kuriyama, Suzuki, Fuse, Kure, Yamashita, Tanabe, Minegishi, Kinoshita, Tsuboi, Shimizu and Yamamoto.
PY - 2021/1/29
Y1 - 2021/1/29
N2 - A baseline oral microbiome study of the Tohoku Medical Megabank Organization (TMM) was planned to characterize the profile of the oral microbiome in the Japanese population. The study also aimed to clarify risk factors for multifactorial diseases by integrated analysis of the oral microbiome and host genome/omics information. From 2013 to 2016, we collected three types of oral biospecimens, saliva, supragingival plaque, and tongue swab, from a total of 25,101 participants who had a dental examination in TMM. In this study, we used two independent cohorts; the Community-Based Cohort and Birth and Three-Generation Cohort as discovery and validation cohorts, respectively, and we selected participants examined by a single dentist. We found through the 16S ribosomal RNA gene sequencing analysis of 834 participants of the Community-Based Cohort Study that there are differences in the microbial composition and community structure between saliva and plaque. The species diversities in both saliva and plaque were increased in correlation with the severity of periodontal disease. These results were nicely reproduced in the analysis of 455 participants of the Birth and Three-Generation Cohort Study. In addition, strong positive and negative associations of microbial taxa in both plaque and saliva with periodontitis-associated biofilm formation were detected by co-occurrence network analysis. The classes Actinobacteria and Bacilli, including oral health-associated bacterial species, showed a positive correlation in saliva. These results revealed differences in microbial composition and community structure between saliva and plaque and a correlation between microbial species and the severity of periodontal disease. We expect that the large database of the oral microbiome in the TMM biobank will help in the discovery of novel targets for the treatment and prevention of oral diseases, as well as for the discovery of therapeutic and/or preventive targets of systemic diseases.
AB - A baseline oral microbiome study of the Tohoku Medical Megabank Organization (TMM) was planned to characterize the profile of the oral microbiome in the Japanese population. The study also aimed to clarify risk factors for multifactorial diseases by integrated analysis of the oral microbiome and host genome/omics information. From 2013 to 2016, we collected three types of oral biospecimens, saliva, supragingival plaque, and tongue swab, from a total of 25,101 participants who had a dental examination in TMM. In this study, we used two independent cohorts; the Community-Based Cohort and Birth and Three-Generation Cohort as discovery and validation cohorts, respectively, and we selected participants examined by a single dentist. We found through the 16S ribosomal RNA gene sequencing analysis of 834 participants of the Community-Based Cohort Study that there are differences in the microbial composition and community structure between saliva and plaque. The species diversities in both saliva and plaque were increased in correlation with the severity of periodontal disease. These results were nicely reproduced in the analysis of 455 participants of the Birth and Three-Generation Cohort Study. In addition, strong positive and negative associations of microbial taxa in both plaque and saliva with periodontitis-associated biofilm formation were detected by co-occurrence network analysis. The classes Actinobacteria and Bacilli, including oral health-associated bacterial species, showed a positive correlation in saliva. These results revealed differences in microbial composition and community structure between saliva and plaque and a correlation between microbial species and the severity of periodontal disease. We expect that the large database of the oral microbiome in the TMM biobank will help in the discovery of novel targets for the treatment and prevention of oral diseases, as well as for the discovery of therapeutic and/or preventive targets of systemic diseases.
KW - 16S ribosomal RNA
KW - dental plaque
KW - microbiome
KW - oral health
KW - periodontal disease
KW - prospective cohort
KW - saliva
UR - http://www.scopus.com/inward/record.url?scp=85100953173&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85100953173&partnerID=8YFLogxK
U2 - 10.3389/fcimb.2020.604596
DO - 10.3389/fcimb.2020.604596
M3 - Article
C2 - 33585276
AN - SCOPUS:85100953173
SN - 2235-2988
VL - 10
JO - Frontiers in cellular and infection microbiology
JF - Frontiers in cellular and infection microbiology
M1 - 604596
ER -
