Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite

Takashi Kumagai; Takahisa Anada; Yoshitomo Honda; Masamichi Takami; Ryutaro Kamijo; Hidetoshi Shimauchi; Osamu Suzuki

Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite

Takashi Kumagai, Takahisa Anada, Yoshitomo Honda, Masamichi Takami, Ryutaro Kamijo, Hidetoshi Shimauchi, Osamu Suzuki

DEN - Dental Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

Original language	English
Pages (from-to)	1025-1028
Number of pages	4
Journal	Key Engineering Materials
Volume	361-363 II
Publication status	Published - 2008

Keywords

BMP-2
Differentiation
Hydroxyapatite
Octacalcium phosphate
Osteoblast

Cite this

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title = "Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite",

abstract = "The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.",

keywords = "BMP-2, Differentiation, Hydroxyapatite, Octacalcium phosphate, Osteoblast",

author = "Takashi Kumagai and Takahisa Anada and Yoshitomo Honda and Masamichi Takami and Ryutaro Kamijo and Hidetoshi Shimauchi and Osamu Suzuki",

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TY - JOUR

T1 - Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite

AU - Kumagai, Takashi

AU - Anada, Takahisa

AU - Honda, Yoshitomo

AU - Takami, Masamichi

AU - Kamijo, Ryutaro

AU - Shimauchi, Hidetoshi

AU - Suzuki, Osamu

PY - 2008

Y1 - 2008

N2 - The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

AB - The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

KW - BMP-2

KW - Differentiation

KW - Hydroxyapatite

KW - Octacalcium phosphate

KW - Osteoblast

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M3 - Article

AN - SCOPUS:38149104640

SN - 1013-9826

VL - 361-363 II

SP - 1025

EP - 1028

JO - Key Engineering Materials

JF - Key Engineering Materials

ER -

Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite

Abstract

Keywords

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