TY - JOUR
T1 - Osteogenesis by gradually expanding the interface between bone surface and periosteum enhanced by bone marrow stem cell administration in rabbits
AU - Sato, Koichiro
AU - Haruyama, Naoto
AU - Shimizu, Yoshinaka
AU - Hara, Junichi
AU - Kawamura, Hiroshi
N1 - Funding Information:
This work was supported by KAKENHI (Grants-in-Aid for Scientific Research; 19659519 to H.K., 19791562 and 19390527 to N.H.).
PY - 2010
Y1 - 2010
N2 - Objective: We investigated whether administration of mesenchymal stem cells (MSCs) promotes bone formation at the gap created by periosteal distraction. Study design: A mesh plate was placed subperiosteally in rabbit parietal bones. Following elevation of the mesh plate, rabbit MSCs were administered into the gap. Controls received phosphate-buffered saline (PBS). The volume, height, bone mineral density (BMD), and bone mineral content (BMC) of newly formed bone were examined using microcomputed tomography. Histological analysis was performed by hematoxylin and eosin staining and immunohistochemistry for type I collagen and osteocalcin. Results: The experimental group showed significantly increased volume, height, BMD, and BMC in newly formed bone tissues at the gaps compared with the control group (P <.05). The newly formed bone tissues showed both type I collagen and osteocalcin expression in the MSC-administration group. Conclusion: Mesenchymal stem cell administration may be useful to induce osteogenesis at sites of periosteal distraction.
AB - Objective: We investigated whether administration of mesenchymal stem cells (MSCs) promotes bone formation at the gap created by periosteal distraction. Study design: A mesh plate was placed subperiosteally in rabbit parietal bones. Following elevation of the mesh plate, rabbit MSCs were administered into the gap. Controls received phosphate-buffered saline (PBS). The volume, height, bone mineral density (BMD), and bone mineral content (BMC) of newly formed bone were examined using microcomputed tomography. Histological analysis was performed by hematoxylin and eosin staining and immunohistochemistry for type I collagen and osteocalcin. Results: The experimental group showed significantly increased volume, height, BMD, and BMC in newly formed bone tissues at the gaps compared with the control group (P <.05). The newly formed bone tissues showed both type I collagen and osteocalcin expression in the MSC-administration group. Conclusion: Mesenchymal stem cell administration may be useful to induce osteogenesis at sites of periosteal distraction.
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U2 - 10.1016/j.tripleo.2009.11.005
DO - 10.1016/j.tripleo.2009.11.005
M3 - Article
C2 - 20188608
AN - SCOPUS:77955304686
SN - 2212-4403
VL - 110
SP - 32
EP - 40
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
IS - 1
ER -