TY - JOUR
T1 - Osteogenic differentiation of bone-marrow-derived stem cells cultured with mixed gelatin and chitooligosaccharide scaffolds
AU - Ratanavaraporn, Juthamas
AU - Damrongsakkul, Siriporn
AU - Kanokpanont, Sorada
AU - Yamamoto, Masaya
AU - Tabata, Yasuhiko
N1 - Funding Information:
Financial support from the Chulalongkorn University Dutsadi Phiphat Scholarship is gratefully acknowledged. The authors wish to thank Tanom Bunaprasert, M.D. for the use of his facilities at i-Tissue Laboratory, Faculty of Medicine, Chula-longkorn University.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - This study investigates the effect of scaffolds prepared from gelatin (G) and chitooligosaccharide (COS) on the osteogenic differentiation of rat bone-marrow-derived mesenchymal stem cells (MSC). The sponge scaffolds at G/COS mixing ratios of 100:0, 70:30 and 50:50 were fabricated by freeze-drying, followed by glutaraldehyde cross-linking. The pore size of the G/COS scaffolds ranged from 70 to 105 μm. MSC cultured in the scaffolds in the osteogenic medium were differentiated into osteogenic cells for all G/COS scaffolds. Calcium nodules were homogeneously formed on the surface of scaffolds cultured with MSC. A Fourier transform infrared (FT-IR) analysis demonstrated the formation of hydroxyapatite spectroscopically. Among all G/COS scaffolds, the highest ALP activity and calcium content were observed for MSC cultured in the G/COS 70:30 scaffolds. The G/COS 70:30 scaffolds were then pre-cultured with MSC in the osteogenic medium for 28 days and subcutaneously implanted into nude mice to evaluate ectopic bone formation. Enhanced vascularization, cell infiltration, collagen formation and calcium deposition around the scaffolds implanted were histologically observed at 2 and 8 weeks after implantation. It was concluded that the G/COS scaffold with the mixing ratio of 70:30 was a promising organic material to induce osteogenic differentiation of MSC.
AB - This study investigates the effect of scaffolds prepared from gelatin (G) and chitooligosaccharide (COS) on the osteogenic differentiation of rat bone-marrow-derived mesenchymal stem cells (MSC). The sponge scaffolds at G/COS mixing ratios of 100:0, 70:30 and 50:50 were fabricated by freeze-drying, followed by glutaraldehyde cross-linking. The pore size of the G/COS scaffolds ranged from 70 to 105 μm. MSC cultured in the scaffolds in the osteogenic medium were differentiated into osteogenic cells for all G/COS scaffolds. Calcium nodules were homogeneously formed on the surface of scaffolds cultured with MSC. A Fourier transform infrared (FT-IR) analysis demonstrated the formation of hydroxyapatite spectroscopically. Among all G/COS scaffolds, the highest ALP activity and calcium content were observed for MSC cultured in the G/COS 70:30 scaffolds. The G/COS 70:30 scaffolds were then pre-cultured with MSC in the osteogenic medium for 28 days and subcutaneously implanted into nude mice to evaluate ectopic bone formation. Enhanced vascularization, cell infiltration, collagen formation and calcium deposition around the scaffolds implanted were histologically observed at 2 and 8 weeks after implantation. It was concluded that the G/COS scaffold with the mixing ratio of 70:30 was a promising organic material to induce osteogenic differentiation of MSC.
KW - Gelatin
KW - bone-marrow-derived stem cells (MSC)
KW - calcium deposition
KW - chitooligosaccharide
KW - ectopic bone formation
KW - osteogenic differentiation
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U2 - 10.1163/092050610X499050
DO - 10.1163/092050610X499050
M3 - Article
C2 - 20615314
AN - SCOPUS:79751514020
SN - 0920-5063
VL - 22
SP - 1083
EP - 1098
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
IS - 8
ER -