In mice, splenic conventional dendritic cells (cDCs) can be separated, based on their expression of CD8α into CD8- and CD8+ cDCs. Although previous experiments demonstrated that injection of antigen (Ag)-pulsed CD8- cDCs into mice induced CD4 T cell differentiation toward Th2 cells, the mechanism involved is unclear. In the current study, we investigated whether OX40 ligand (OX40L) on CD8- cDCs contributes to the induction of Th2 responses by Ag-pulsed CD8- cDCs in vivo, because OX40-OX40L interactions may play a preferential role in Th2 cell development. When unseparated Ag-pulsed OX40L-deficient cDCs were injected into syngeneic BALB/c mice, Th2 cytokine (IL-4, IL-5, and IL-10) production in lymph node cells was significantly reduced. Splenic cDCs were separated to CD8 - and CD8+ cDCs. OX40L expression was not observed on freshly isolated CD8- cDCs, but was induced by anti-CD40 mAb stimulation for 24 h. Administration of neutralizing anti-OX40L mAb significantly inhibited IL-4, IL-5, and IL-10 production induced by Ag-pulsed CD8- cDC injection. Moreover, administration of anti-OX40L mAb with Ag-pulsed CD8- cDCs during a secondary response also significantly inhibited Th2 cytokine production. Thus, OX40L on CD8- cDCs physiologically contributes to the development of Th2 cells and secondary Th2 responses induced by Ag-pulsed CD8- cDCs in vivo.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2014 Feb 7|
- Dendritic cell
- OX40 ligand
- Th2 response