TY - GEN
T1 - Oxygen metabolism analysis of a vascularized spheroid using a scanning electrochemical microscopy
AU - Nashimoto, Yuji
AU - Mukomoto, Rei
AU - Terai, Takato
AU - Ino, Kosuke
AU - Nishiyama, Koichi
AU - Yokokawa, Ryuji
AU - Miura, Takashi
AU - Shiku, Hitoshi
N1 - Funding Information:
This work is mainly supported by JST CREST, JSPS, Asahi Glass Foundation, and Tokyo Ohka Foundation.
Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - We investigated the effect of vascularization on oxygen metabolism in a fibroblast spheroid. By the scanning of a microelectrode, the oxygen reduction current was monitored, which allowed us to analyze the oxygen metabolism quantitatively. The oxygen metabolism of the spheroids were determined by the spheroid radius (volume) and did not depend on whether the spheroid was vascularized or not, implying the identical activity of oxygen metabolism of the fibroblast and endothelial cells. Our system is useful for the metabolic evaluation of a three-dimensional culture model.
AB - We investigated the effect of vascularization on oxygen metabolism in a fibroblast spheroid. By the scanning of a microelectrode, the oxygen reduction current was monitored, which allowed us to analyze the oxygen metabolism quantitatively. The oxygen metabolism of the spheroids were determined by the spheroid radius (volume) and did not depend on whether the spheroid was vascularized or not, implying the identical activity of oxygen metabolism of the fibroblast and endothelial cells. Our system is useful for the metabolic evaluation of a three-dimensional culture model.
KW - Electrochemical analysis
KW - Oxygen metabolism
KW - Three-dimensional culture
KW - Vascularization
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M3 - Conference contribution
AN - SCOPUS:85098257420
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 520
EP - 521
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -