Panton–valentíne leukocidin genes in a phage-like particle isolated from mitomycin C-Treated staphylococcus aureus V8 (ATCC 49775)

Jun Kaneko; Takahiro Kimura; Yoshiyuki Kawakami; Toshio Tomita; Yoshiyuki Kamio

doi:10.1271/bbb.61.1960

Panton–valentíne leukocidin genes in a phage-like particle isolated from mitomycin C-Treated staphylococcus aureus V8 (ATCC 49775)

Jun Kaneko, Takahiro Kimura, Yoshiyuki Kawakami, Toshio Tomita, Yoshiyuki Kamio

AGR - Agricultural Chemistry

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

The staphylococcal Panton–Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (ϕPVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in ϕPVL was a linear double-stranded 40-kb DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase géne) of ϕPVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus.

Original language	English
Pages (from-to)	1960-1962
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	11
DOIs	https://doi.org/10.1271/bbb.61.1960
Publication status	Published - 1997 Jan

Keywords

Carrying Panton-Valentine leukocidin genes
Integrase
LukF-PV
Luks-PV
Staphylococcus aureus V8 (ATCC 49775)
Temperate phage (πPVL)

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10.1271/bbb.61.1960

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title = "Panton–valent{\'i}ne leukocidin genes in a phage-like particle isolated from mitomycin C-Treated staphylococcus aureus V8 (ATCC 49775)",

abstract = "The staphylococcal Panton–Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (ϕPVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in ϕPVL was a linear double-stranded 40-kb DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase g{\'e}ne) of ϕPVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus.",

keywords = "Carrying Panton-Valentine leukocidin genes, Integrase, LukF-PV, Luks-PV, Staphylococcus aureus V8 (ATCC 49775), Temperate phage (πPVL)",

author = "Jun Kaneko and Takahiro Kimura and Yoshiyuki Kawakami and Toshio Tomita and Yoshiyuki Kamio",

note = "Funding Information: Acknowledgments, We are grateful to Mr. Tsuruji Sato for his technical assistance in electron microscopy. This work was supported by in part by Grants-in-Aid for Scientific Research (No. 08307004, No. 8660086, and No.09460042) from the Ministry of Education, Science. Sports, and Culture of Japan.",

year = "1997",

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doi = "10.1271/bbb.61.1960",

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AU - Tomita, Toshio

AU - Kamio, Yoshiyuki

N1 - Funding Information: Acknowledgments, We are grateful to Mr. Tsuruji Sato for his technical assistance in electron microscopy. This work was supported by in part by Grants-in-Aid for Scientific Research (No. 08307004, No. 8660086, and No.09460042) from the Ministry of Education, Science. Sports, and Culture of Japan.

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N2 - The staphylococcal Panton–Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (ϕPVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in ϕPVL was a linear double-stranded 40-kb DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase géne) of ϕPVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus.

AB - The staphylococcal Panton–Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (ϕPVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in ϕPVL was a linear double-stranded 40-kb DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase géne) of ϕPVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus.

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KW - Temperate phage (πPVL)

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Panton–valentíne leukocidin genes in a phage-like particle isolated from mitomycin C-Treated staphylococcus aureus V8 (ATCC 49775)

Abstract

Keywords

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