Partial reconstitution of the photosynthetic oxygen evolution system by rebinding of the 33-kDa polypeptide

Mitsue Miyao; Norio Murata

doi:10.1016/0014-5793(83)80320-2

Partial reconstitution of the photosynthetic oxygen evolution system by rebinding of the 33-kDa polypeptide

Mitsue Miyao, Norio Murata

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Treatment with 2.6 M urea of the Photosystem II particles depleted of two polypeptides of 24 kDa and 18 kDa completely released a polypeptide of 33 kDa and eliminated the oxygen-evolution activity. The 33-kDa polypeptide rebound to the urea-treated particles and partially reactivated the oxygen evolution. A quantitative analysis of the rebinding suggests tha there is a specific binding site for the 33-kDa polypeptide on the membrane surface.

Original language	English
Pages (from-to)	375-378
Number of pages	4
Journal	FEBS Letters
Volume	164
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(83)80320-2
Publication status	Published - 1983 Dec 12
Externally published	Yes

Keywords

(Spinach chloroplast)
33-kDa polypeptide
Oxygen evolution
Photosynthesis
Photosystem II

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(83)80320-2

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@article{d01bdbb165914c8fae5df15cbd29f71c,

title = "Partial reconstitution of the photosynthetic oxygen evolution system by rebinding of the 33-kDa polypeptide",

abstract = "Treatment with 2.6 M urea of the Photosystem II particles depleted of two polypeptides of 24 kDa and 18 kDa completely released a polypeptide of 33 kDa and eliminated the oxygen-evolution activity. The 33-kDa polypeptide rebound to the urea-treated particles and partially reactivated the oxygen evolution. A quantitative analysis of the rebinding suggests tha there is a specific binding site for the 33-kDa polypeptide on the membrane surface.",

keywords = "(Spinach chloroplast), 33-kDa polypeptide, Oxygen evolution, Photosynthesis, Photosystem II",

author = "Mitsue Miyao and Norio Murata",

note = "Funding Information: This work is supportedb y Grants-in-Aid for Energy Research( 56040054a) nd for Cooperative Research( 58340037t)o N.M. from the Japanese Ministry of Education, Sciencea nd Culture.",

year = "1983",

month = dec,

day = "12",

doi = "10.1016/0014-5793(83)80320-2",

language = "English",

volume = "164",

pages = "375--378",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

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TY - JOUR

T1 - Partial reconstitution of the photosynthetic oxygen evolution system by rebinding of the 33-kDa polypeptide

AU - Miyao, Mitsue

AU - Murata, Norio

N1 - Funding Information: This work is supportedb y Grants-in-Aid for Energy Research( 56040054a) nd for Cooperative Research( 58340037t)o N.M. from the Japanese Ministry of Education, Sciencea nd Culture.

PY - 1983/12/12

Y1 - 1983/12/12

N2 - Treatment with 2.6 M urea of the Photosystem II particles depleted of two polypeptides of 24 kDa and 18 kDa completely released a polypeptide of 33 kDa and eliminated the oxygen-evolution activity. The 33-kDa polypeptide rebound to the urea-treated particles and partially reactivated the oxygen evolution. A quantitative analysis of the rebinding suggests tha there is a specific binding site for the 33-kDa polypeptide on the membrane surface.

AB - Treatment with 2.6 M urea of the Photosystem II particles depleted of two polypeptides of 24 kDa and 18 kDa completely released a polypeptide of 33 kDa and eliminated the oxygen-evolution activity. The 33-kDa polypeptide rebound to the urea-treated particles and partially reactivated the oxygen evolution. A quantitative analysis of the rebinding suggests tha there is a specific binding site for the 33-kDa polypeptide on the membrane surface.

KW - (Spinach chloroplast)

KW - 33-kDa polypeptide

KW - Oxygen evolution

KW - Photosynthesis

KW - Photosystem II

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U2 - 10.1016/0014-5793(83)80320-2

DO - 10.1016/0014-5793(83)80320-2

M3 - Article

AN - SCOPUS:0039243690

SN - 0014-5793

VL - 164

SP - 375

EP - 378

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Partial reconstitution of the photosynthetic oxygen evolution system by rebinding of the 33-kDa polypeptide

Abstract

Keywords

ASJC Scopus subject areas

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