Patterning the surface cytophobicity of an albumin-physisorbed substrate by electrochemical means

Hirokazu Kaji, Kazuto Tsukidate, Masahiko Hashimoto, Tomokazu Matsue, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Spatiotemporal control of surface properties under physiological conditions such as those found in culture media is an important technique in fundamental cell biology, tissue engineering, and cell-based bioelectronics. To this end, we have developed a mild, wet cellular micropatterning technique. The principle of the technique is based on the fact that the cell-repellant property of the albumin-coated substrate rapidly switches to cell-adhesive upon exposure to the reactive oxidizing agent, electrochemically generated hypobromous acid. Herein, we report the effect of the hypobromous acid on serum albumin physisorbed on a hydrophobic substrate. It was found that albumin molecules detach from the substrate by application of the oxidizing agent, resulting in exposure of the underlying hydrophobic surface to the liquid phase. The adsorption of extracellular matrix proteins such as fibronectin onto the hydrophobic surface induces cell adhesion and growth.

Original languageEnglish
Pages (from-to)6966-6969
Number of pages4
Issue number15
Publication statusPublished - 2005 Jul 19


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