PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens

Rathina Kumar Shanmugakani; Yukihiro Akeda; Norihisa Yamamoto; Noriko Sakamoto; Hideharu Hagiya; Hisao Yoshida; Dan Takeuchi; Yo Sugawara; Takuya Kodera; Mitsuo Kawase; Warawut Laolerd; Narong Chaihongsa; Pitak Santanirand; Yoshikazu Ishii; Shigeyuki Hamada; Kazunori Tomono

doi:10.1128/AAC.00067-17

PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens

Rathina Kumar Shanmugakani, Yukihiro Akeda, Norihisa Yamamoto, Noriko Sakamoto, Hideharu Hagiya, Hisao Yoshida, Dan Takeuchi, Yo Sugawara, Takuya Kodera, Mitsuo Kawase, Warawut Laolerd, Narong Chaihongsa, Pitak Santanirand, Yoshikazu Ishii, Shigeyuki Hamada, Kazunori Tomono

MEN - Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.

Original language	English
Article number	e00067-17
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	6
DOIs	https://doi.org/10.1128/AAC.00067-17
Publication status	Published - 2017 Jun

Keywords

Carbapenemase
Enterobacteriaceae
Molecular diagnostics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.00067-17

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Shanmugakani, R. K., Akeda, Y., Yamamoto, N., Sakamoto, N., Hagiya, H., Yoshida, H., Takeuchi, D., Sugawara, Y., Kodera, T., Kawase, M., Laolerd, W., Chaihongsa, N., Santanirand, P., Ishii, Y., Hamada, S., & Tomono, K. (2017). PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens. Antimicrobial Agents and Chemotherapy, 61(6), Article e00067-17. https://doi.org/10.1128/AAC.00067-17

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abstract = "A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.",

keywords = "Carbapenemase, Enterobacteriaceae, Molecular diagnostics",

author = "Shanmugakani, {Rathina Kumar} and Yukihiro Akeda and Norihisa Yamamoto and Noriko Sakamoto and Hideharu Hagiya and Hisao Yoshida and Dan Takeuchi and Yo Sugawara and Takuya Kodera and Mitsuo Kawase and Warawut Laolerd and Narong Chaihongsa and Pitak Santanirand and Yoshikazu Ishii and Shigeyuki Hamada and Kazunori Tomono",

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Shanmugakani, RK, Akeda, Y, Yamamoto, N, Sakamoto, N, Hagiya, H, Yoshida, H, Takeuchi, D, Sugawara, Y, Kodera, T, Kawase, M, Laolerd, W, Chaihongsa, N, Santanirand, P, Ishii, Y, Hamada, S & Tomono, K 2017, 'PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens', Antimicrobial Agents and Chemotherapy, vol. 61, no. 6, e00067-17. https://doi.org/10.1128/AAC.00067-17

TY - JOUR

T1 - PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens

AU - Shanmugakani, Rathina Kumar

AU - Akeda, Yukihiro

AU - Yamamoto, Norihisa

AU - Sakamoto, Noriko

AU - Hagiya, Hideharu

AU - Yoshida, Hisao

AU - Takeuchi, Dan

AU - Sugawara, Yo

AU - Kodera, Takuya

AU - Kawase, Mitsuo

AU - Laolerd, Warawut

AU - Chaihongsa, Narong

AU - Santanirand, Pitak

AU - Ishii, Yoshikazu

AU - Hamada, Shigeyuki

AU - Tomono, Kazunori

PY - 2017/6

Y1 - 2017/6

N2 - A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.

AB - A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.

KW - Carbapenemase

KW - Enterobacteriaceae

KW - Molecular diagnostics

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ER -

PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens

Abstract

Keywords

UN SDGs

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