Perfluorocarbon-Based 19F MRI Nanoprobes for In Vivo Multicolor Imaging

Kazuki Akazawa, Fuminori Sugihara, Tatsuya Nakamura, Hisashi Matsushita, Hiroaki Mukai, Rena Akimoto, Masafumi Minoshima, Shin Mizukami, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


In vivo multicolor imaging is important for monitoring multiple biomolecular or cellular processes in biology. 19F magnetic resonance imaging (MRI) is an emerging in vivo imaging technique because it can non-invasively visualize 19F nuclei without endogenous background signals. Therefore, 19F MRI probes capable of multicolor imaging are in high demand. Herein, we report five types of perfluorocarbon-encapsulated silica nanoparticles that show 19F NMR peaks with different chemical shifts. Three of the nanoprobes, which show spectrally distinct 19F NMR peaks with sufficient sensitivity, were selected for in vivo multicolor 19F MRI. The nanoprobes exhibited 19F MRI signals with three colors in a living mouse. Our in vivo multicolor system could be utilized for evaluating the effect of surface functional groups on the hepatic uptake in a mouse. This novel multicolor imaging technology will be a practical tool for elucidating in vivo biomolecular networks by 19F MRI.

Original languageEnglish
Pages (from-to)16742-16747
Number of pages6
JournalAngewandte Chemie - International Edition
Issue number51
Publication statusPublished - 2018 Dec 17


  • fluorine
  • imaging agents
  • magnetic resonance imaging
  • multicolor imaging
  • nanoparticles


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