@article{e2022c131f9549afb4f0d65e90cf7cdd,
title = "Perfluorocarbon-Based 19F MRI Nanoprobes for In Vivo Multicolor Imaging",
abstract = "In vivo multicolor imaging is important for monitoring multiple biomolecular or cellular processes in biology. 19F magnetic resonance imaging (MRI) is an emerging in vivo imaging technique because it can non-invasively visualize 19F nuclei without endogenous background signals. Therefore, 19F MRI probes capable of multicolor imaging are in high demand. Herein, we report five types of perfluorocarbon-encapsulated silica nanoparticles that show 19F NMR peaks with different chemical shifts. Three of the nanoprobes, which show spectrally distinct 19F NMR peaks with sufficient sensitivity, were selected for in vivo multicolor 19F MRI. The nanoprobes exhibited 19F MRI signals with three colors in a living mouse. Our in vivo multicolor system could be utilized for evaluating the effect of surface functional groups on the hepatic uptake in a mouse. This novel multicolor imaging technology will be a practical tool for elucidating in vivo biomolecular networks by 19F MRI.",
keywords = "fluorine, imaging agents, magnetic resonance imaging, multicolor imaging, nanoparticles",
author = "Kazuki Akazawa and Fuminori Sugihara and Tatsuya Nakamura and Hisashi Matsushita and Hiroaki Mukai and Rena Akimoto and Masafumi Minoshima and Shin Mizukami and Kazuya Kikuchi",
note = "Funding Information: We thank Dr. Takao Sakata (the Research Center for Ultra-High Voltage Electron Microscopy, Osaka University) for his support in TEM measurements. We are also grateful to Prof. Masaru Ishii, Dr. Junichi Kikuta, and Dr. Hiroki Mizuno (Graduate School of Medicine, Osaka University) for providing the RAW264.7 cells. This research was supported by the Grant-in-Aid for Scientific Research (Grant No. 25220207, 18H03935, and 16K01933), and Innovative Areas “Frontier Research on Chemical Communications” (No. 17H06409) of MEXT, Japan; JSPS A3 Foresight Program; JSPS Asian CORE Program, “Asian Chemical Biology Initiative”; and the Magnetic Health Science Foundation. Funding Information: We thank Dr. Takao Sakata (the Research Center for UltraHigh Voltage Electron Microscopy, Osaka University) for his support in TEM measurements. We are also grateful to Prof. Masaru Ishii, Dr. Junichi Kikuta, and Dr. Hiroki Mizuno (Graduate School of Medicine, Osaka University) for providing the RAW264.7 cells. This research was supported by the Grant-in-Aid for Scientific Research (Grant No. 25220207, 18H03935, and 16K01933), and Innovative Areas “Frontier Research on Chemical Communications” (No. 17H06409) of MEXT, Japan; JSPS A3 Foresight Program; JSPS Asian CORE Program, “Asian Chemical Biology Initiative”; and the Magnetic Health Science Foundation. Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2018",
month = dec,
day = "17",
doi = "10.1002/anie.201810363",
language = "English",
volume = "57",
pages = "16742--16747",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "51",
}