Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry

Tomokazu Matsue; Hitoshi Shiku; Hiroshi Yamada; Isamu Uchida

doi:10.1021/j100094a002

Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry

Tomokazu Matsue, Hitoshi Shiku, Hiroshi Yamada, Isamu Uchida

Tohoku University

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The permeability of Ru(NH₃)₆³⁺, Fe(CN)₆^3-, and I^- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)₆^3- was ca. 10 times smaller than that of Ru(NH₃)₆³⁺ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.

Original language	English
Pages (from-to)	11001-11003
Number of pages	3
Journal	Journal of Physical Chemistry
Volume	98
Issue number	43
DOIs	https://doi.org/10.1021/j100094a002
Publication status	Published - 1994

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title = "Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry",

abstract = "The permeability of Ru(NH3)63+, Fe(CN)63-, and I- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)63- was ca. 10 times smaller than that of Ru(NH3)63+ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.",

author = "Tomokazu Matsue and Hitoshi Shiku and Hiroshi Yamada and Isamu Uchida",

year = "1994",

doi = "10.1021/j100094a002",

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journal = "Journal of Physical Chemistry",

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T1 - Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry

AU - Matsue, Tomokazu

AU - Shiku, Hitoshi

AU - Yamada, Hiroshi

AU - Uchida, Isamu

PY - 1994

Y1 - 1994

N2 - The permeability of Ru(NH3)63+, Fe(CN)63-, and I- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)63- was ca. 10 times smaller than that of Ru(NH3)63+ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.

AB - The permeability of Ru(NH3)63+, Fe(CN)63-, and I- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)63- was ca. 10 times smaller than that of Ru(NH3)63+ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.

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JO - Journal of Physical Chemistry

JF - Journal of Physical Chemistry

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Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry

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