We synthesized [18F]FCWAY, an analog of [carbonyl-11C]WAY-100635 ([11C]N-(2-(1-(4-(2-methoxyphenyl)-piperazinyl)ethyl))-N-(2- (pyridinyl))cyclohexanecarboxamide), by replacing the cyclohexanecarbonyl group acid with a trans-4-fluorocyclohexanecarbonyl group (FC). Control and preblocking studies were performed in anesthetized monkeys. Plasma radioactive metabolite analysis showed the presence of [18F]FC and [18F]fluoride. Tissue time-radioactivity curves were corrected for metabolite contamination based on separate positron-emission tomography studies of these two labeled metabolites. Analysis using a two-tissue compartment model gave distribution volume (V) estimates (mL/mL) ranging from 33 in frontal cortex to 4 in cerebellum. Preblocking data showed uniform V of 2-3 mL/mL. These studies demonstrate that [18F]FCWAY has very similar kinetic characteristics to [11C]WAY-100635.
- 5-HT receptors
- Volume of distribution