Pharmacological characterization of micropatterned cardiac myocytes

Hirokazu Kaji; Yuki Takii; Matsuhiko Nishizawa; Tomokazu Matsue

doi:10.1016/S0142-9612(03)00275-8

Pharmacological characterization of micropatterned cardiac myocytes

Hirokazu Kaji, Yuki Takii, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The cardiac myocytes patterned on a single cell level were prepared by microcontact printing, and the response to chemical stimuli was studied using confocal fluorescent Ca²⁺ imaging. The patterned myocytes were found to conjugate by forming gap junction. It was confirmed for the patterned myocytes that gap junction communication was reversibly inhibited by 1-octanol, but activated by caffeine. Localized stimulation with chemicals was also attempted using a microinjection system for the myocyte patterns formed by single cell alignment. This research was carried out with the objective of developing a bioassay system based on a cellular network.

Original language	English
Pages (from-to)	4239-4244
Number of pages	6
Journal	Biomaterials
Volume	24
Issue number	23
DOIs	https://doi.org/10.1016/S0142-9612(03)00275-8
Publication status	Published - 2003 Oct

Keywords

Cardiomyocyte
Cell adhesion
Fibronectin
Micropatterning
Polydimethylsiloxane

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/S0142-9612(03)00275-8

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title = "Pharmacological characterization of micropatterned cardiac myocytes",

abstract = "The cardiac myocytes patterned on a single cell level were prepared by microcontact printing, and the response to chemical stimuli was studied using confocal fluorescent Ca2+ imaging. The patterned myocytes were found to conjugate by forming gap junction. It was confirmed for the patterned myocytes that gap junction communication was reversibly inhibited by 1-octanol, but activated by caffeine. Localized stimulation with chemicals was also attempted using a microinjection system for the myocyte patterns formed by single cell alignment. This research was carried out with the objective of developing a bioassay system based on a cellular network.",

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author = "Hirokazu Kaji and Yuki Takii and Matsuhiko Nishizawa and Tomokazu Matsue",

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T1 - Pharmacological characterization of micropatterned cardiac myocytes

AU - Kaji, Hirokazu

AU - Takii, Yuki

AU - Nishizawa, Matsuhiko

AU - Matsue, Tomokazu

N1 - Funding Information: The present work was supported by Grants-in-Aid for Scientific Research on Priority Area (No. 1122720) and B (No. 13450348) from the Ministry of Education, Science and Culture, Japan.

PY - 2003/10

Y1 - 2003/10

N2 - The cardiac myocytes patterned on a single cell level were prepared by microcontact printing, and the response to chemical stimuli was studied using confocal fluorescent Ca2+ imaging. The patterned myocytes were found to conjugate by forming gap junction. It was confirmed for the patterned myocytes that gap junction communication was reversibly inhibited by 1-octanol, but activated by caffeine. Localized stimulation with chemicals was also attempted using a microinjection system for the myocyte patterns formed by single cell alignment. This research was carried out with the objective of developing a bioassay system based on a cellular network.

AB - The cardiac myocytes patterned on a single cell level were prepared by microcontact printing, and the response to chemical stimuli was studied using confocal fluorescent Ca2+ imaging. The patterned myocytes were found to conjugate by forming gap junction. It was confirmed for the patterned myocytes that gap junction communication was reversibly inhibited by 1-octanol, but activated by caffeine. Localized stimulation with chemicals was also attempted using a microinjection system for the myocyte patterns formed by single cell alignment. This research was carried out with the objective of developing a bioassay system based on a cellular network.

KW - Cardiomyocyte

KW - Cell adhesion

KW - Fibronectin

KW - Micropatterning

KW - Polydimethylsiloxane

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Pharmacological characterization of micropatterned cardiac myocytes

Abstract

Keywords

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