Phenobarbital molecularly imprinted polymer selectively binds phenobarbital

Yoshihisa Tomioka; Yoshiki Kudo; Tetsuro Hayashi; Hitoshi Nakamura; Masahiro Niizeki; Takanori Hishinuma; Michinao Mizugaki

doi:10.1248/bpb.20.397

Phenobarbital molecularly imprinted polymer selectively binds phenobarbital

Yoshihisa Tomioka, Yoshiki Kudo, Tetsuro Hayashi, Hitoshi Nakamura, Masahiro Niizeki, Takanori Hishinuma, Michinao Mizugaki

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Molecularly imprinted polymer (MIP) was prepared against phenobarbital using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking monomer. We analyzed the recognition properties of the phenobarbital MIP. In some organic solvents, imprinted polymer showed selective binding to phenobarbital. Two dissociation constants of binding were calculated by Scatchard plot analyses; K(d) values were 1.8, 121.7 μM, and the number of binding sites was 8.3, 92.3 μmol/g MIP in toluene-heptane-acetic acid (25:75:1, v/v), respectively. The relationship between the binding affinity to phenobarbital MIP and the polarity of the solvent system, as well as the structure of the template molecule is also discussed.

Original language	English
Pages (from-to)	397-400
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	20
Issue number	4
DOIs	https://doi.org/10.1248/bpb.20.397
Publication status	Published - 1997 Apr

Keywords

antibody mimic
molecular imprinting
molecular recognition
molecularly imprinted polymer (MIP)
phenobarbital

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

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abstract = "Molecularly imprinted polymer (MIP) was prepared against phenobarbital using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking monomer. We analyzed the recognition properties of the phenobarbital MIP. In some organic solvents, imprinted polymer showed selective binding to phenobarbital. Two dissociation constants of binding were calculated by Scatchard plot analyses; K(d) values were 1.8, 121.7 μM, and the number of binding sites was 8.3, 92.3 μmol/g MIP in toluene-heptane-acetic acid (25:75:1, v/v), respectively. The relationship between the binding affinity to phenobarbital MIP and the polarity of the solvent system, as well as the structure of the template molecule is also discussed.",

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author = "Yoshihisa Tomioka and Yoshiki Kudo and Tetsuro Hayashi and Hitoshi Nakamura and Masahiro Niizeki and Takanori Hishinuma and Michinao Mizugaki",

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AU - Tomioka, Yoshihisa

AU - Kudo, Yoshiki

AU - Hayashi, Tetsuro

AU - Nakamura, Hitoshi

AU - Niizeki, Masahiro

AU - Hishinuma, Takanori

AU - Mizugaki, Michinao

PY - 1997/4

Y1 - 1997/4

N2 - Molecularly imprinted polymer (MIP) was prepared against phenobarbital using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking monomer. We analyzed the recognition properties of the phenobarbital MIP. In some organic solvents, imprinted polymer showed selective binding to phenobarbital. Two dissociation constants of binding were calculated by Scatchard plot analyses; K(d) values were 1.8, 121.7 μM, and the number of binding sites was 8.3, 92.3 μmol/g MIP in toluene-heptane-acetic acid (25:75:1, v/v), respectively. The relationship between the binding affinity to phenobarbital MIP and the polarity of the solvent system, as well as the structure of the template molecule is also discussed.

AB - Molecularly imprinted polymer (MIP) was prepared against phenobarbital using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking monomer. We analyzed the recognition properties of the phenobarbital MIP. In some organic solvents, imprinted polymer showed selective binding to phenobarbital. Two dissociation constants of binding were calculated by Scatchard plot analyses; K(d) values were 1.8, 121.7 μM, and the number of binding sites was 8.3, 92.3 μmol/g MIP in toluene-heptane-acetic acid (25:75:1, v/v), respectively. The relationship between the binding affinity to phenobarbital MIP and the polarity of the solvent system, as well as the structure of the template molecule is also discussed.

KW - antibody mimic

KW - molecular imprinting

KW - molecular recognition

KW - molecularly imprinted polymer (MIP)

KW - phenobarbital

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ER -

Phenobarbital molecularly imprinted polymer selectively binds phenobarbital

Abstract

Keywords

ASJC Scopus subject areas

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