Phenotypic analysis of a novel chordin mutant in medaka

Shigeo Takashima, Atsuko Shimada, Daisuke Kobayashi, Hayato Yokoi, Takanori Narita, Tomoko Jindo, Takahiro Kage, Tadao Kitagawa, Tetsuaki Kimura, Koshin Sekimizu, Akimitsu Miyake, Davin H.E. Setiamarga, Ryohei Murakami, Sachiko Tsuda, Shinya Ooki, Ken Kakihara, Motoki Hojo, Kiyoshi Naruse, Hiroshi Mitani, Akihiro ShimaYuji Ishikawa, Kazuo Araki, Yumiko Saga, Hiroyuki Takeda

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


We have isolated and characterized a ventralized mutant in medaka (the Japanese killifish; Oryzias latipes), which turned out to have a mutation in the chordin gene. The mutant exhibits ventralization of the body axis, malformation of axial bones, over-bifurcation of yolk sac blood vessels, and laterality defects in internal organs. The mutant exhibits variability of phenotypes, depending on the culture temperature, from embryos with a slightly ventralized phenotype to those without any head and trunk structures. Taking advantages of these variable and severe phenotypes, we analyzed the role of Chordin-dependent tissues such as the notochord and Kupffer's vesicle (KV) in the establishment of left-right axis in fish. The results demonstrate that, in the absence of the notochord and KV, the medaka lateral plate mesoderm autonomously and bilaterally expresses spaw gene in a default state.

Original languageEnglish
Pages (from-to)2298-2310
Number of pages13
JournalDevelopmental Dynamics
Issue number8
Publication statusPublished - 2007 Aug


  • BMP
  • Chordin
  • Dorsal-ventral patterning
  • Kupffer's vesicle
  • Medaka


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