Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1

Akira Asai, Fumitaka Okajima, Kiyotaka Nakagawa, Daigo Ibusuki, Kyoko Tanimura, Yasushi Nakajima, Mototsugu Nagao, Mariko Sudo, Taro Harada, Teruo Miyazawa, Shinichi Oikawa

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


The accumulation of phosphatidylcholine hydroperoxide (PCOOH), a primary oxidation product of phosphatidylcholine (PC), in blood plasma and tissues has been observed in various pathological conditions, including atherosclerosis. However, the biological roles of PCOOH in these conditions remain unknown. To estimate the atherogenicity of PCOOH, we evaluated the effect of PCOOH on THP-1 monocytic cell adherence to immobilized vascular endothelial cell adhesion molecules. THP-1 cell adhesion to intracellular adhesion molecule-1 (ICAM-1) was dose-dependently increased by addition of PCOOH. Phosphatidylcholine hydroxide (a hydroxyl analog of PCOOH) also induced THP-1 cell adhesion to ICAM-1, whereas nonoxidized PC, sn-2 truncated PCs, and other hydroperoxide compounds did not affect the adhesion. In the PCOOH-treated cells, obvious protruding F-actin-rich membrane structures were formed, and lymphocyte function-associated antigen-1 (LFA-1) was localized to the protruding structures. Cytochalasin D, an actin polymerization inhibitor, suppressed the PCOOH-induced cell adhesion to ICAM-1 and the membrane protrusions. These results indicate that PCOOH evokes LFA-1-mediated cell adhesion to ICAM-1 via actin cytoskeletal organization, and the mechanism may participate in monocyte adherence to the arterial wall in the initiation of atherosclerosis.

Original languageEnglish
Pages (from-to)957-965
Number of pages9
JournalJournal of Lipid Research
Issue number5
Publication statusPublished - 2009 May


  • Actin cytoskeleton
  • Atherosclerosis
  • Cell adhesion molecule
  • Integrin
  • Lipid oxidation
  • Monocyte
  • Phospholipid


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