(Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics.
|Number of pages||4|
|Journal||Angewandte Chemie - International Edition|
|Publication status||Published - 2005 Jun 6|
- Affinity purification
- Molecular targeting
ASJC Scopus subject areas