Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor β and γ chains

Nobuyuki Tanaka; Hironobu Asao; Kazuyuki Ohbo; Naoto Ishii; Toshikazu Takeshita; Masataka Nakamura; Hidetada Sasaki; Kazuo Sugamura

doi:10.1073/pnas.91.15.7271

Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor β and γ chains

Nobuyuki Tanaka, Hironobu Asao, Kazuyuki Ohbo, Naoto Ishii, Toshikazu Takeshita, Masataka Nakamura, Hidetada Sasaki, Kazuo Sugamura

MED - Medical Sciences

Tohoku University

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

The functional interleukin 2 (IL-2) receptors contain the β and γ chains which are necessary for the transduction of cell growth signals. Monoclonal antibodies specific for the β chain and γ chain coimmunoprecipitated JAK1 and 114-kDa JAK2 tyrosine kinases, respectively. Tyrosine phosphorylation of JAK1 and JAK2 was induced upon IL-2 stimulation, and IL-2 activated the JAK2 kinase. These results demonstrate that the JAK1 and JAK2 tyrosine kinases are physically associated with the β chain and γ chain, respectively, and suggest that regulation of the kinases may be linked to IL-2-induced signal transduction.

Original language	English
Pages (from-to)	7271-7275
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	15
DOIs	https://doi.org/10.1073/pnas.91.15.7271
Publication status	Published - 1994 Jul 19

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abstract = "The functional interleukin 2 (IL-2) receptors contain the β and γ chains which are necessary for the transduction of cell growth signals. Monoclonal antibodies specific for the β chain and γ chain coimmunoprecipitated JAK1 and 114-kDa JAK2 tyrosine kinases, respectively. Tyrosine phosphorylation of JAK1 and JAK2 was induced upon IL-2 stimulation, and IL-2 activated the JAK2 kinase. These results demonstrate that the JAK1 and JAK2 tyrosine kinases are physically associated with the β chain and γ chain, respectively, and suggest that regulation of the kinases may be linked to IL-2-induced signal transduction.",

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T1 - Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor β and γ chains

AU - Tanaka, Nobuyuki

AU - Asao, Hironobu

AU - Ohbo, Kazuyuki

AU - Ishii, Naoto

AU - Takeshita, Toshikazu

AU - Nakamura, Masataka

AU - Sasaki, Hidetada

AU - Sugamura, Kazuo

PY - 1994/7/19

Y1 - 1994/7/19

N2 - The functional interleukin 2 (IL-2) receptors contain the β and γ chains which are necessary for the transduction of cell growth signals. Monoclonal antibodies specific for the β chain and γ chain coimmunoprecipitated JAK1 and 114-kDa JAK2 tyrosine kinases, respectively. Tyrosine phosphorylation of JAK1 and JAK2 was induced upon IL-2 stimulation, and IL-2 activated the JAK2 kinase. These results demonstrate that the JAK1 and JAK2 tyrosine kinases are physically associated with the β chain and γ chain, respectively, and suggest that regulation of the kinases may be linked to IL-2-induced signal transduction.

AB - The functional interleukin 2 (IL-2) receptors contain the β and γ chains which are necessary for the transduction of cell growth signals. Monoclonal antibodies specific for the β chain and γ chain coimmunoprecipitated JAK1 and 114-kDa JAK2 tyrosine kinases, respectively. Tyrosine phosphorylation of JAK1 and JAK2 was induced upon IL-2 stimulation, and IL-2 activated the JAK2 kinase. These results demonstrate that the JAK1 and JAK2 tyrosine kinases are physically associated with the β chain and γ chain, respectively, and suggest that regulation of the kinases may be linked to IL-2-induced signal transduction.

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Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor β and γ chains

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