PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis

Mika Inoue; Makio Saeki; Hiroshi Egusa; Hitoshi Niwa; Yoshinori Kamisaki

doi:10.1016/j.bbrc.2010.11.031

PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis

Mika Inoue, Makio Saeki, Hiroshi Egusa, Hitoshi Niwa, Yoshinori Kamisaki

DEN - Dental Sciences

Osaka University

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1D1 transcripts were abundant in lung, leukocyte, and placenta. The reduction in endogenous PIH1D1 by siRNA enhanced apoptosis and caspase-3 activation induced by doxorubicin in U2OS cells. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway.

Original language	English
Pages (from-to)	340-344
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	403
Issue number	3-4
DOIs	https://doi.org/10.1016/j.bbrc.2010.11.031
Publication status	Published - 2010 Dec 17

Keywords

Apoptosis
Monad
PIH1D1
R2TP
Reptin
RPAP3
WDR92

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10.1016/j.bbrc.2010.11.031

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title = "PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis",

abstract = "We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1D1 transcripts were abundant in lung, leukocyte, and placenta. The reduction in endogenous PIH1D1 by siRNA enhanced apoptosis and caspase-3 activation induced by doxorubicin in U2OS cells. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway.",

keywords = "Apoptosis, Monad, PIH1D1, R2TP, Reptin, RPAP3, WDR92",

author = "Mika Inoue and Makio Saeki and Hiroshi Egusa and Hitoshi Niwa and Yoshinori Kamisaki",

note = "Funding Information: This study was supported in part by Japan Society for the Promotion of Science Grants C20592172 (M.S.), B20390471 (Y.K.), and B22390364 (H.E.) and the Osaka Medical Research Foundation for Incurable Diseases.",

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language = "English",

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T1 - PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis

AU - Inoue, Mika

AU - Saeki, Makio

AU - Egusa, Hiroshi

AU - Niwa, Hitoshi

AU - Kamisaki, Yoshinori

N1 - Funding Information: This study was supported in part by Japan Society for the Promotion of Science Grants C20592172 (M.S.), B20390471 (Y.K.), and B22390364 (H.E.) and the Osaka Medical Research Foundation for Incurable Diseases.

PY - 2010/12/17

Y1 - 2010/12/17

N2 - We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1D1 transcripts were abundant in lung, leukocyte, and placenta. The reduction in endogenous PIH1D1 by siRNA enhanced apoptosis and caspase-3 activation induced by doxorubicin in U2OS cells. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway.

AB - We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1D1 transcripts were abundant in lung, leukocyte, and placenta. The reduction in endogenous PIH1D1 by siRNA enhanced apoptosis and caspase-3 activation induced by doxorubicin in U2OS cells. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway.

KW - Apoptosis

KW - Monad

KW - PIH1D1

KW - R2TP

KW - Reptin

KW - RPAP3

KW - WDR92

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DO - 10.1016/j.bbrc.2010.11.031

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SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3-4

ER -

PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis

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Keywords

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