TY - JOUR
T1 - PMab-213
T2 - A Monoclonal Antibody for Immunohistochemical Analysis Against Pig Podoplanin
AU - Kato, Yukinari
AU - Yamada, Shinji
AU - Furusawa, Yoshikazu
AU - Itai, Shunsuke
AU - Nakamura, Takuro
AU - Yanaka, Miyuki
AU - Sano, Masato
AU - Harada, Hiroyuki
AU - Fukui, Masato
AU - Kaneko, Mika K.
N1 - Funding Information:
This research was supported in part by AMED under Grant Numbers: JP18am0101078 (Y.K.), JP18am0301010 (Y.K.), and JP18ae0101028 (Y.K.), and by JSPS KAKENHI Grant Number 17K07299 (M.K.K.) and Grant Number 16K10748 (Y.K.).
Publisher Copyright:
© 2019, Mary Ann Liebert, Inc., publishers.
PY - 2019/2
Y1 - 2019/2
N2 - Podoplanin (PDPN) is known to be expressed in normal tissues, including lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPN have already been established; however, mAbs against pig PDPN (pPDPN) are lacking. In the present study, mice were immunized with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and hybridomas producing mAbs against pPDPN were identified by flow cytometric screening. One of the mAbs, PMab-213 (IgG 2b , kappa), could specifically detect CHO/pPDPN cells through flow cytometry and detect pPDPN through western blot analysis. K D of PMab-213 for CHO/pPDPN was determined to be 2.1 × 10 -9 M, indicating a high affinity for CHO/pPDPN. Furthermore, PMab-213 strongly stained lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells through immunohistochemistry. PMab-213 is expected to be useful in investigating the function of pPDPN.
AB - Podoplanin (PDPN) is known to be expressed in normal tissues, including lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPN have already been established; however, mAbs against pig PDPN (pPDPN) are lacking. In the present study, mice were immunized with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and hybridomas producing mAbs against pPDPN were identified by flow cytometric screening. One of the mAbs, PMab-213 (IgG 2b , kappa), could specifically detect CHO/pPDPN cells through flow cytometry and detect pPDPN through western blot analysis. K D of PMab-213 for CHO/pPDPN was determined to be 2.1 × 10 -9 M, indicating a high affinity for CHO/pPDPN. Furthermore, PMab-213 strongly stained lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells through immunohistochemistry. PMab-213 is expected to be useful in investigating the function of pPDPN.
KW - PDPN
KW - PMab-213
KW - Pig podoplanin
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U2 - 10.1089/mab.2018.0048
DO - 10.1089/mab.2018.0048
M3 - Article
C2 - 30802179
AN - SCOPUS:85062182433
SN - 2167-9436
VL - 38
SP - 18
EP - 24
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 1
ER -