PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas

Mika K. Kaneko; Ryusuke Honma; Satoshi Ogasawara; Yuki Fujii; Takuro Nakamura; Noriko Saidoh; Michiaki Takagi; Yumiko Kagawa; Satoru Konnai; Yukinari Kato

doi:10.1089/mab.2016.0036

PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas

Mika K. Kaneko, Ryusuke Honma, Satoshi Ogasawara, Yuki Fujii, Takuro Nakamura, Noriko Saidoh, Michiaki Takagi, Yumiko Kagawa, Satoru Konnai, Yukinari Kato

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Podoplanin, a type I transmembrane protein, is expressed in lymphatic endothelial cells. Although we previously developed an anticanine podoplanin monoclonal antibody (mAb), PMab-38, immunohistochemistry (IHC) showed that it did not react with canine lymphatic endothelial cells. Here, we determined whether PMab-38 recognizes canine podoplanin of squamous cell carcinomas (SCCs) and clarified its epitope. In IHC, PMab-38 reacted with 83% of SCCs (15/18 cases). Flow cytometry showed that the epitope of PMab-38 was different from that of the platelet aggregation-stimulating domain of the N-terminus, which was detected by almost all antipodoplanin mAbs such as D2-40 or NZ-1. PMab-38 is expected to be useful for investigating the function of podoplanin in canine tumors.

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume	35
Issue number	5
DOIs	https://doi.org/10.1089/mab.2016.0036
Publication status	Published - 2016 Oct

Keywords

canine podoplanin
immunohistochemistry
monoclonal antibody

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title = "PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas",

abstract = "Podoplanin, a type I transmembrane protein, is expressed in lymphatic endothelial cells. Although we previously developed an anticanine podoplanin monoclonal antibody (mAb), PMab-38, immunohistochemistry (IHC) showed that it did not react with canine lymphatic endothelial cells. Here, we determined whether PMab-38 recognizes canine podoplanin of squamous cell carcinomas (SCCs) and clarified its epitope. In IHC, PMab-38 reacted with 83% of SCCs (15/18 cases). Flow cytometry showed that the epitope of PMab-38 was different from that of the platelet aggregation-stimulating domain of the N-terminus, which was detected by almost all antipodoplanin mAbs such as D2-40 or NZ-1. PMab-38 is expected to be useful for investigating the function of podoplanin in canine tumors.",

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author = "Kaneko, {Mika K.} and Ryusuke Honma and Satoshi Ogasawara and Yuki Fujii and Takuro Nakamura and Noriko Saidoh and Michiaki Takagi and Yumiko Kagawa and Satoru Konnai and Yukinari Kato",

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T1 - PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas

AU - Kaneko, Mika K.

AU - Honma, Ryusuke

AU - Ogasawara, Satoshi

AU - Fujii, Yuki

AU - Nakamura, Takuro

AU - Saidoh, Noriko

AU - Takagi, Michiaki

AU - Kagawa, Yumiko

AU - Konnai, Satoru

AU - Kato, Yukinari

PY - 2016/10

Y1 - 2016/10

N2 - Podoplanin, a type I transmembrane protein, is expressed in lymphatic endothelial cells. Although we previously developed an anticanine podoplanin monoclonal antibody (mAb), PMab-38, immunohistochemistry (IHC) showed that it did not react with canine lymphatic endothelial cells. Here, we determined whether PMab-38 recognizes canine podoplanin of squamous cell carcinomas (SCCs) and clarified its epitope. In IHC, PMab-38 reacted with 83% of SCCs (15/18 cases). Flow cytometry showed that the epitope of PMab-38 was different from that of the platelet aggregation-stimulating domain of the N-terminus, which was detected by almost all antipodoplanin mAbs such as D2-40 or NZ-1. PMab-38 is expected to be useful for investigating the function of podoplanin in canine tumors.

AB - Podoplanin, a type I transmembrane protein, is expressed in lymphatic endothelial cells. Although we previously developed an anticanine podoplanin monoclonal antibody (mAb), PMab-38, immunohistochemistry (IHC) showed that it did not react with canine lymphatic endothelial cells. Here, we determined whether PMab-38 recognizes canine podoplanin of squamous cell carcinomas (SCCs) and clarified its epitope. In IHC, PMab-38 reacted with 83% of SCCs (15/18 cases). Flow cytometry showed that the epitope of PMab-38 was different from that of the platelet aggregation-stimulating domain of the N-terminus, which was detected by almost all antipodoplanin mAbs such as D2-40 or NZ-1. PMab-38 is expected to be useful for investigating the function of podoplanin in canine tumors.

KW - canine podoplanin

KW - immunohistochemistry

KW - monoclonal antibody

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JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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IS - 5

ER -

PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas

Abstract

Keywords

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