TY - JOUR
T1 - PMab-44 Detects Bovine Podoplanin in Immunohistochemistry
AU - Honma, Ryusuke
AU - Ogasawara, Satoshi
AU - Kaneko, Mika K.
AU - Fujii, Yuki
AU - Oki, Hiroharu
AU - Nakamura, Takuro
AU - Takagi, Michiaki
AU - Konnai, Satoru
AU - Kato, Yukinari
N1 - Funding Information:
We thank Noriko Saidoh and Kanae Yoshida for their excellent technical assistance. This work was supported, in part, by the Regional Innovation Strategy Support Program from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Y.K.), by JSPS KAKENHI Grant Number 26440019 (M.K.K.), Grant Number 25462242 (Y.K.), and Grant Number 16K10748 (Y.K.) by Takeda Science Foundation (S.O.), by the Platform for Drug Discovery, Informatics, and Structural Life Science (PDIS) from Japan Agency for Medical Research and development, AMED (Y.K.), and by the Basic Science and Platform Technology Program for Innovative Biological Medicine from AMED (Y.K.). This work was performed, in part, under the Co-operative Research Program of Institute for Protein Research, Osaka University, CR-15-05 and CR-16-05.
Publisher Copyright:
© 2016, Mary Ann Liebert, Inc.
PY - 2016/8
Y1 - 2016/8
N2 - Podoplanin (PDPN)/Aggrus is a type I transmembrane O-glycoprotein, which is expressed in several normal tissues including podocytes of kidney and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet; however, only bovine PDPN (bovPDPN) does not possess the platelet-aggregating activity. Although many monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, and rabbit PDPN have been established, anti-bovPDPN mAbs have not been developed. In this study, we immunized mice with the recombinant proteins of bovPDPN and developed anti-bovPDPN mAbs, which are useful in immunohistochemical analysis. One of the clones, PMab-44, is useful for detecting podocytes and lymphatic endothelial cells in normal bovine tissues. PMab-44 also detected bovPDPN specifically in flow cytometry. PMab-44 is expected to be useful for investigating the function of bovPDPN.
AB - Podoplanin (PDPN)/Aggrus is a type I transmembrane O-glycoprotein, which is expressed in several normal tissues including podocytes of kidney and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet; however, only bovine PDPN (bovPDPN) does not possess the platelet-aggregating activity. Although many monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, and rabbit PDPN have been established, anti-bovPDPN mAbs have not been developed. In this study, we immunized mice with the recombinant proteins of bovPDPN and developed anti-bovPDPN mAbs, which are useful in immunohistochemical analysis. One of the clones, PMab-44, is useful for detecting podocytes and lymphatic endothelial cells in normal bovine tissues. PMab-44 also detected bovPDPN specifically in flow cytometry. PMab-44 is expected to be useful for investigating the function of bovPDPN.
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U2 - 10.1089/mab.2016.0016
DO - 10.1089/mab.2016.0016
M3 - Article
AN - SCOPUS:84993941196
SN - 2167-9436
VL - 35
SP - 186
EP - 190
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 4
ER -