TY - JOUR
T1 - Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus
AU - Maekawa, Takaki
AU - Kusakabe, Mitsumasa
AU - Shimoda, Yoshikazu
AU - Sato, Shusei
AU - Tabata, Satoshi
AU - Murooka, Yoshikatsu
AU - Hayashi, Makoto
PY - 2008/4
Y1 - 2008/4
N2 - In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
AB - In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
UR - http://www.scopus.com/inward/record.url?scp=42049099904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42049099904&partnerID=8YFLogxK
U2 - 10.1094/MPMI-21-4-0375
DO - 10.1094/MPMI-21-4-0375
M3 - Article
C2 - 18321183
AN - SCOPUS:42049099904
SN - 0894-0282
VL - 21
SP - 375
EP - 382
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 4
ER -