Population doublings of murine CD4+ memory T cells during continuous antigen stimulation in vivo

Yoshihiro Kushida, Jun Ya Ishida, Masato Fujii, Maki Touma, Masamichi Hosono

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


We investigated the expansion rate of CD4+ memory T cells using a newly developed in vivo system. Neonatal thymectomy abrogates the subsequent production of T cells and induces autoimmune gastritis (AIG) by the activation of CD4+ T cells; this disease was transferred into athymic nude mice through the inoculation of splenic CD4+ memory T cells. The transferred CD4+ T cells increased logarithmically in number during the first 2months in the spleen of the recipients. The serial transfer of these splenocytes at two-month intervals revealed that the numbers of the AIG-transferable generations were inversely correlated with the age of the first AIG donors. The duration of the AIG-promoting capacity of CD4+ T cells under continuous antigenic stimulation in vivo was approximately equivalent-one and a half years. These results indicate that there exists an intrinsic population doubling limit in memory CD4+ T cells similar to that of self-renewing naïve ones.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalCellular Immunology
Issue number1
Publication statusPublished - 2014 Dec 1


  • Autoimmune gastritis
  • In vivo culture
  • Memory CD4 T cells
  • Population doublings
  • Serial transfer


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