TY - JOUR
T1 - Porous nanosheet wrapping for live imaging of suspension cells
AU - Zhang, Hong
AU - Aoki, Takuto
AU - Hatano, Kanae
AU - Kabayama, Kazuya
AU - Nakagawa, Masaru
AU - Fukase, Koichi
AU - Okamura, Yosuke
N1 - Funding Information:
The authors wish to thank JVCKENWOOD Creative Media Co. Ltd for providing nickel molds for nanoimprinting, and Technical Service Coordination Office, Imaging Center for Advanced Research at Tokai University for technical assistance. This work is supported in part by a Cooperative Research Program of ‘‘Network Joint Research Center for Materials and Devices’’ (No. 2015096) (Y. O.), a Grant-in-Aid for Scientific Research on Innovative Areas ‘‘Nanomedicine Molecular Science’’ (No. 2306) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) (Y. O.), and a MEXT-Supported Program for the Strategic Research Foundation at Private Universities (Y. O.).
Publisher Copyright:
© 2018 The Royal Society of Chemistry.
PY - 2018
Y1 - 2018
N2 - In the field of cell imaging, it is still a practical challenge to obtain the high quality live imaging of suspension cells, mainly due to undesirable cell movement in the imaging field during observation. This study describes a porous nanosheet wrapping method to noninvasively immobilize suspension cells for their live imaging. Perforated nanopores are fabricated on a nanosheet to enable the addition of external chemicals to cells, ranging from small molecules to macromolecules. Through several case studies, such as the live imaging of membrane staining of liposomes, transferrin endocytosis of B cells, and activation of platelets, it is verified that the confined space made by the nanosheet could provide a hydrodynamically stable environment for suspension cells, even if an aqueous stimulus is added through the nanopores in a static or a flowing condition. With this method, the live imaging of the whole activation process on a specific suspension cell in the imaging field is achieved, which is not feasible with the existing cell immobilization methods. This study suggests that the method of porous nanosheet wrapping will facilitate the visualization of the dynamic functions of suspension cells.
AB - In the field of cell imaging, it is still a practical challenge to obtain the high quality live imaging of suspension cells, mainly due to undesirable cell movement in the imaging field during observation. This study describes a porous nanosheet wrapping method to noninvasively immobilize suspension cells for their live imaging. Perforated nanopores are fabricated on a nanosheet to enable the addition of external chemicals to cells, ranging from small molecules to macromolecules. Through several case studies, such as the live imaging of membrane staining of liposomes, transferrin endocytosis of B cells, and activation of platelets, it is verified that the confined space made by the nanosheet could provide a hydrodynamically stable environment for suspension cells, even if an aqueous stimulus is added through the nanopores in a static or a flowing condition. With this method, the live imaging of the whole activation process on a specific suspension cell in the imaging field is achieved, which is not feasible with the existing cell immobilization methods. This study suggests that the method of porous nanosheet wrapping will facilitate the visualization of the dynamic functions of suspension cells.
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U2 - 10.1039/c8tb01943f
DO - 10.1039/c8tb01943f
M3 - Article
C2 - 31999284
AN - SCOPUS:85055701737
SN - 2050-7518
VL - 6
SP - 6622
EP - 6628
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 41
ER -