Porphyromonas gingivalis fimbriae induce adhesion of monocytic cell line U937 to endothelial cells

Kimiharu Hirose, Emiko Isogai, Itsuo Ueda

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


This study used the human monocytic cell line U937 to examine whether or not Porphyromonas gingivalis fimbriae could induce the adhesion of monocytes to endothelial cells. An in vitro adhesion assay was used to investigate the effects of the fimbriae on U937 cell adhesion to human umbilical vein endothelial cells (HUVEC). The fimbriae enhanced U937 cell adhesion to HUVEC in a dose-dependent manner. U937 cells adhered better to HUVEC pretreated with the fimbriae for a minimum of 2 hr than to untreated HUVEC. The enhanced adhesion was inhibited by a monoclonal antibody against P. gingivalis 381 fimbriae. Pretreatment of U937 cells with the fimbriae for 24 hr enhanced U937 cell adhesion to HUVEC approximately 4-fold. This phenomenon was inhibited by an anti-CD11b antibody, suggesting the involvement of CD11b. These results indicate that P. gingivalis fimbriae can induce monocyte adhesion to the endothelial cell surface. They also suggest that the fimbriae may be involved in the initial event for infiltration of monocytes into the periodontal tissues of individuals with adult periodontitis.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
Issue number1
Publication statusPublished - 2000
Externally publishedYes


  • Adhesion
  • Endothelial cells
  • Monocyte
  • Porphyromonas gingivalis fimbriae

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology


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