Possible involvement of rho-kinase in aldosterone-induced vascular smooth muscle cell remodeling

Kayoko Miyata, Hirofumi Hitomi, Peng Guo, Guo Xing Zhang, Shoji Kimura, Hideyasu Kiyomoto, Naohisa Hosomi, Shoji Kagami, Masakazu Kohno, Akira Nishiyama

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


There is increasing evidence supporting potential roles of aldosterone in the pathogenesis of vascular injury. The present study aimed to determine the involvement of Rho-kinase in aldosterone-induced vascular smooth muscle cell (VSMC) remodeling. In cultured rat VSMC, the effects of aldosterone on Rho-kinase activity, the reorganization of the cytoskeleton and cellular migration were examined. Aldosterone (1 nmol/ L) significantly increased phosphorylation of myosin phosphate target subunit-1 (MYPT1), a marker of Rhokinase activity, and the amount of GTP-Rho with a peak at 90 min in VSMC. Aldosterone also stimulated VSMC stress fiber formation and migration. These effects of aldosterone were markedly attenuated by pretreatment with eplerenone (10 μmol/L), a selective mineralocorticoid receptor antagonist, or Y27632 (10 μmol/ L), a specific Rho-kinase inhibitor. These findings indicate that Rho-kinase is involved in the pathogenesis of aldosterone-induced VSMC remodeling.

Original languageEnglish
Pages (from-to)1407-1413
Number of pages7
JournalHypertension Research
Issue number7
Publication statusPublished - 2008


  • Aldosterone
  • Eplerenone
  • Mineralocorticoid receptor
  • Rho-kinase
  • Vascular smooth muscle cells

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine


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