Postantifungal-like effect of sublethal treatment of Candida albicans with acid-electrolyzed water

Takayuki Mokudai, Taro Kanno, Yoshimi Niwano

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Objective Acid-electrolyzed water (AEW) has been applied to the treatment of oral candidiasis. We evaluated the postantifungal effect (PAFE)-like activity of AEW against Candida albicans under sublethal conditions by exposing C. albicans to dilute AEW.

Design The growth of C. albicans after a short-term exposure to dilute AEW was evaluated in broth and on agar culture. The involvement of reactive oxygen species (ROS) in the PAFE was examined by flow cytometric analysis with hydroxyphenyl fluorescein (HPF) as a fluorescence probe.

Results The dilute AEW exerted PAFE-like activity against C. albicans. ROS were produced in the cells treated with AEW diluted 16 times or fewer. The increase in HPF fluorescence after treatment with dilute AEW was cancelled by dimethyl sulfoxide, a hydroxyl radical (OH) scavenger.

Conclusion It would be expected that the ROS, especially OH, produced in the C. albicans cells treated with sublethal dilutions of AEW could exert PAFE-like activity against the fungal cells.

Original languageEnglish
Pages (from-to)479-487
Number of pages9
JournalArchives of Oral Biology
Issue number3
Publication statusPublished - 2015 Mar

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology


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