Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model

Kazuya Sakai, Yuki Hiradate, Kenshiro Hara, Kentaro Tanemura

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1 Citation (Scopus)

Abstract

Testicular toxicity is a major concern in cancer chemotherapy and drug development as it can result in infertility; however, there are no effective biomarkers for this adverse effect. To identify new biomarkers, we investigated the expression of small non-coding RNAs (sncRNAs) in a mouse model of doxorubicin (DXR)-induced testicular toxicity. First, we performed small RNA-seq analysis of sperm from DXR-treated or control mice and observed differential expression of many genome-derived sequences. We then performed real-time RT-PCR validation of these sequences and discovered that sncRNA detected by one primers, dxRN_3, showed similar differential expression as that seen in the RNA-seq experiment. These findings suggest that the sncRNAs present in sperm have potential as clinically acceptable biomarkers for testicular toxicity.

Original languageEnglish
Article number101160
JournalBiochemistry and Biophysics Reports
Volume28
DOIs
Publication statusPublished - 2021 Dec

Keywords

  • Biomarkers
  • Doxorubicin
  • Drug development and chemotherapy
  • RNA-Sequencing
  • Small non-coding RNAs
  • Testicular toxicity

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